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International Journal of Pharmacology
  Year: 2019 | Volume: 15 | Issue: 2 | Page No.: 156-165
DOI: 10.3923/ijp.2019.156.165
1-Hydroxy-3,7,8-Trimethoxyxanthone Suppresses the Malignant Proliferation of Human Bone Marrow Mesenchymal Stem Cells in Colon Cancer Microenvironment
Hui-qiao Zhao, Nian-hua Lu, Xu-dong Zhang, Na Liu and Ming Jing

Abstract:
Background and Objective: Human bone marrow mesenchymal stem cells (HMSCs) have been widely used to study tumor gene therapy. However, they may be induced malignant proliferation in some cancers. Therefore, how to decrease the side effects of HMSCs has been becoming a hot topic. In this study, the role of 1-hydroxy-3,7,8-trimethoxyxanthone (HTX) on HMSCs in colon cancer microenvironment in vitro was evaluated. Materials and Methods: Non-contact co-culture system of colon cancer cells SW480 and HMSCs was established using Transwell cell culture chambers. 20, 10, 5 μg mL1 of HTX was used to treat HMSCs in the colon cancer microenvironment. The proliferation of HMSCs in each group was tested using the MTT assay. The activity of HMSCs in each group was tested using Transwell invasion and migration assays. RT-PCR assay, Western Blot assay and ELISA assay were used to detect the expression of related genes and proteins. Results: In colon cancer microenvironment, the proliferation, invasion and migration of HMSCs significantly increased, however, supplemented with HTX isolated from Tibetan medicine Gentianopsis paludosa significantly improved the side effects. Furthermore, HTX inhibited the expression of IL-6 and the phosphorylation of STAT3 signalling pathway in HMSCs which was reduced by colon cancer microenvironment. Conclusion: All the results indicated that HTX inhibited the malignant proliferation of HMSCs in colon cancer microenvironment by inhibiting IL-6/STAT3 signaling pathway. The HTX may be a novel chemical inhibitor for gene therapy of colon cancer with HMSCs as carrier.
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How to cite this article:

Hui-qiao Zhao, Nian-hua Lu, Xu-dong Zhang, Na Liu and Ming Jing, 2019. 1-Hydroxy-3,7,8-Trimethoxyxanthone Suppresses the Malignant Proliferation of Human Bone Marrow Mesenchymal Stem Cells in Colon Cancer Microenvironment. International Journal of Pharmacology, 15: 156-165.

DOI: 10.3923/ijp.2019.156.165

URL: https://scialert.net/abstract/?doi=ijp.2019.156.165

 
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