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International Journal of Pharmacology

Year: 2018 | Volume: 14 | Issue: 6 | Page No.: 873-878
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Authors

Hui Peng

Xiaoqiang Zhuang

Yu Bai

Xu Fang

Hui Huang

Shenglin Lu

Shenglin Lu

Keywords

Research Article

Alteration of Cellular and Humoral Immunity by the Blockage of P2y11 Gene Attenuates on the Rheumatoid Arthritis

Hui Peng, Xiaoqiang Zhuang, Yu Bai, Xu Fang, Hui Huang and Shenglin Lu Shenglin Lu's LiveDNA
Background and Objective: P2Y11 is a sub type of purinergic receptors that altered the function of immune cells. Present study evaluates the beneficial effect of P2Y11 receptor blockage on rheumatoid arthritis (RA). Methodology: Arthritis was induced by the intraplantar injecting the mixture of complete freund’s adjuvant (CFA) and type II collagen in left hind paw. P2Y11 receptor was blocked by P2Y11 receptor antagonist (NF340). All the animals were separated in to five groups like control group, negative group (Rheumatoid arthritis group), NF340 0.3, 10 and 30 μM/day injected intrathecally for the period of 21 days. Development of RA was estimated by arthritic score, paw swelling and paw withdrawal latency. Pro-inflammatory cytokines in the synovial fluid, anti-type II collagen antibodies in serum and proliferation of T-cell was estimated in RA rats. Results: Data of the study reveals that treatment with NF340 attenuates the development of RA. There was significant (p<0.01) decrease in the pro-inflammatory cytokines in NF340 treated group compared to negative control group. Moreover treatment with NF340 significantly reduced anti-type II collagen antibodies and increase in the proliferation of T-cells compared to negative control group. Conclusion: Present investigation proves that gene expression of P2Y11 receptor blocked by NF340 attenuates the RA by ameliorating the cellular and humoral immunity.
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