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International Journal of Pharmacology
  Year: 2018 | Volume: 14 | Issue: 5 | Page No.: 689-697
DOI: 10.3923/ijp.2018.689.697
Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-health
Xiande Ma, Yan Jin and Hongquan Guan

Abstract:
Background and Objective: Sub-health, also known as yajiankang, is mainly caused by fatigue and an unbalanced diet and has become a serious threat to public health. This study aimed to evaluate the susceptibility and innate immune response to intestinal Candida albicans (C. albicans) infection in mice induced by fatigue combined with an unbalanced diet as a sub-health state model. Materials and Methods: The KM mice were randomly divided into 4 groups: control group (CG), control mice infected with C. albicans group (CCG), model group (MG) and model mice infected with C. albicans group (MCG). The C. albicans content in feces was counted and barrier function analysis was performed on the small intestine. The levels of sIgA, IL-1β and TNF-α were determined by enzyme-linked immunosorbent assay. Expression of lysozyme was determined by Western blot. Results: These results indicated that C. albicans content was significantly increased, but the sIgA level, phagocytic percentage and index, 50% hemolytic complement (as CH50 level) and expression of lysozyme were significantly decreased in the mice infected with C. albicans group (MCG) relative to those in the other groups. The IL-1β and TNF-α levels were significantly decreased in the MCG relative to those in the control mice infected with C. albicans group. In the barrier function analysis, inflammatory cells in the mucosa, sparse microvilli and vacuolar phenomenon were observed in the MCG. Conclusion: The results indicated that innate immunity was down regulated and susceptibility to C. albicans infection was significantly higher in mice in the sub-health state than in control mice.
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How to cite this article:

Xiande Ma, Yan Jin and Hongquan Guan, 2018. Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-health. International Journal of Pharmacology, 14: 689-697.

DOI: 10.3923/ijp.2018.689.697

URL: https://scialert.net/abstract/?doi=ijp.2018.689.697

 
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