Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2018 | Volume: 14 | Issue: 5 | Page No.: 652-666
DOI: 10.3923/ijp.2018.652.666
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail
Flavonoids-rich Extract of Beta vulgaris Subsp. cicla L. var. Flavescens Leaf, a Promising Protector Against Gentamicin- induced Nephrotoxicity and Hepatotoxicity in Rats
Sherien Kamal Hassan , Nermin Mohammed El-Sammad , Abeer Hamed Abdel-Halim, Amria Mamdouh Mousa , Wagdy Khalil Bassaly Khalil and Nayera Anwar

Abstract:
Background and Objective: The major complication of gentamicin antibiotic is nephrotoxicity which limited its clinical use. The current study was conducted to evaluate the possible protective effect of Beta vulgaris L. subsp. cicla var. flavescens (Swiss chard) against gentamicin-induced nephrotoxicity and hepatotoxicity in rats. Methodology: Twenty four rats were divided into 4 groups, group 1served as control. Group 2 to group 4 were intraperitoneally (i.p.) injected with gentamicin at a dose of 80 mg kg–1 body weight daily for 15 days. Swiss chard extract was orally administrated 1 week before and along with gentamicin treatment to groups 3 and 4 at doses of 300 and 600 mg kg–1 body weight daily, respectively. Results: The results revealed that, gentamicin significantly altered serum levels of kidney and liver markers as well as tumor necrosis factor alpha (TNF-α). These results were associated with significant changes in urinary urea, creatinine, micro-total protein (Micro TP) and vascular epithelial growth factor (VEGF) levels. A significant decrease in renal and hepatic reduced glutathione (GSH) levels, glutathione peroxidase (GSH-Px), catalase (CAT) and superoxide dismutase (SOD) enzymatic activities with a significant decrease in the expression levels of SOD1 and GSH-Px genes were also observed. In contrary, a significant increase in malondialdehyde (MDA), protein carbonyl levels and caspase-3 gene expression levels were also detected in gentamicin treated rats. Pretreatment with Swiss chard, dose dependently, ameliorated such altered changes. In harmony with this line, Swiss chard greatly decreased the severity of renal tubular and hepatic necrosis induced by gentamicin. Conclusion: Swiss chard leaf extract can attenuate gentamicin-induced nephrotoxicity and hepatotoxicity possibly by its antioxidant, anti-inflammatory and anti-apoptotic properties.
PDF Fulltext XML References Citation Report Citation
How to cite this article:

Sherien Kamal Hassan, Nermin Mohammed El-Sammad, Abeer Hamed Abdel-Halim, Amria Mamdouh Mousa, Wagdy Khalil Bassaly Khalil and Nayera Anwar, 2018. Flavonoids-rich Extract of Beta vulgaris Subsp. cicla L. var. Flavescens Leaf, a Promising Protector Against Gentamicin- induced Nephrotoxicity and Hepatotoxicity in Rats. International Journal of Pharmacology, 14: 652-666.

DOI: 10.3923/ijp.2018.652.666

URL: https://scialert.net/abstract/?doi=ijp.2018.652.666

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom