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International Journal of Pharmacology
  Year: 2018 | Volume: 14 | Issue: 3 | Page No.: 310-319
DOI: 10.3923/ijp.2018.310.319
MicroRNA Microarray Analysis to Investigate the Key Genes and microRNAs Regulated by Amomum cardamomum in Nephropathy Rat Model
M.M. Hongmei Chen, M.D. Xiulan Wang , M.M. Enhesuren, B.M. Chang Chun, B.M. Wenjie Jin and B.M. Mengqiqige

Abstract:
Background and Objective: Nephropathy is a chronic non-communicable disease that can result in serious consequences. As the dried fruit of Zingiberaceae plant, Amomum cardamomum can improve renal function. This study aimed to reveal the action mechanisms of Amomum cardamomum in nephropathy rats. Materials and Methods: After nephropathy rat model was established, nephropathy rats were divided into model and drug groups (treated with Amomum cardamomum). The microRNA (miRNA) expression profilings of the rats were generated and the differentially expressed miRNAs (DE-miRNAs) between control and model groups were analyzed using the limma package. The DE-miRNAs between drug and model groups were also analyzed and then their targets were predicted by the miRWalk2.0 tool. Using DAVID tool, functional enrichment analysis for target genes was performed. In addition, protein-protein interaction (PPI) network and module analyses were conducted for target genes and miRNA-miRNA co-regulatory network was also constructed. Results: The nephropathy rat model was successfully established. A total of 21 DE-miRNAs were identified in drug group compared with model group. In the PPI networks, TP53, AKT2, HDAC1 and STAT3 had higher degrees. Besides, TP53 could interact with AKT2 and HDAC1. Moreover, STAT3 was co-regulated by rno-miR-30a-3p and rno-miR-30e-5p. Additionally, functional enrichment analysis showed that rno-miR-30a-3p-rno-miR-30e-5p, rno-miR-195-3p-rno-miR-32-3p separately had synergistic effects. Conclusion: Amomum cardamomum might improve the renal function of nephropathy rats by regulating TP53, AKT2, HDAC1, STAT3, miR-30a-5p, miR-30e-5p and miR-195-3p.
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How to cite this article:

M.M. Hongmei Chen, M.D. Xiulan Wang, M.M. Enhesuren, B.M. Chang Chun, B.M. Wenjie Jin and B.M. Mengqiqige, 2018. MicroRNA Microarray Analysis to Investigate the Key Genes and microRNAs Regulated by Amomum cardamomum in Nephropathy Rat Model. International Journal of Pharmacology, 14: 310-319.

DOI: 10.3923/ijp.2018.310.319

URL: https://scialert.net/abstract/?doi=ijp.2018.310.319

 
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