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International Journal of Pharmacology
  Year: 2018 | Volume: 14 | Issue: 8 | Page No.: 1169-1178
DOI: 10.3923/ijp.2018.1169.1178
 
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Effectiveness of Osthole on Uric Acid Crystal-induced Acute Gouty Arthritis Through the Inhibition of NLRP3 Inflammasome
Ming Yang, Gaopeng Teng, Guangjian Li, Tao Huang and Renguo Xu

Abstract:
Background and Objective: Inflammation is a key factor in the pathogenesis of arthritis. In the present study, investigated the potential of osthole, a major natural product, to treat monosodium urate crystal (MSU) induced acute gouty arthritis in rats and explore the mechanisms underlying osthole mediated immunomodulation. Materials and Methods: Rats were oral administrated with osthole at 24 h prior to 3 mg MSU injection, the volume and width of swelling ankle were recorded to evaluate the protection of osthole in vivo. LPS+MSU in Raw 264.7 cells was utilized to assess the effects of osthole on NLRP3 inflammasome in vitro. Results: Osthole could alleviate MSU-induced arthritis through inhibiting the generation of inflammatory factors and NLRP3 inflammasome activation in vivo. Pretreatment with osthole significantly suppressed MSU or LPS induced NF-κB signal along with the transcription of inflammatory factors. In addition, osthole decreased MSU induced oxidative stress and lysosomal damage. Current findings illustrated that osthole significantly suppressed NLRP3 inflammasome in synovial tissue and macrophage cells. The potential mechanism may be based on the attenuation of NF-κB mediated first signal and oxidative stress and lysosome mediated second signal in NLRP3 inflammasome activation. Conclusion: Osthole might be a promising therapeutic agent for alleviating gouty arthritis through inhibiting NLRP3 inflammasome.
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How to cite this article:

Ming Yang, Gaopeng Teng, Guangjian Li, Tao Huang and Renguo Xu, 2018. Effectiveness of Osthole on Uric Acid Crystal-induced Acute Gouty Arthritis Through the Inhibition of NLRP3 Inflammasome. International Journal of Pharmacology, 14: 1169-1178.

DOI: 10.3923/ijp.2018.1169.1178

URL: https://scialert.net/abstract/?doi=ijp.2018.1169.1178

 
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