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International Journal of Pharmacology
  Year: 2018 | Volume: 14 | Issue: 8 | Page No.: 1087-1098
DOI: 10.3923/ijp.2018.1087.1098
Evaluation of Anticoagulant and Antithrombotic Activities of Berberine: A Focus on the Ameliorative Effect on Blood Hypercoagulation
Can Wang, Yan-Bin Wu, Ai-Ping Wang, Jian-Dong Jiang and Wei-Jia Kong

Abstract:
Background and Objective: Thrombosis and related diseases are the leading causes of mortality and disability worldwide. This study aimed to investigate the anticoagulant and antithrombotic activities of natural product berberine (BBR) using in vitro and in vivo models. Materials and Methods: In in vitro experiments, BBR was used to treat venous blood or plasma isolated from rabbits, with heparin sodium (HS) as a positive control. In the animal experiments, BBR was orally administered to Wistar rats fed with a regular diet or a high-fat diet (HFD) for 2 or 4 weeks, respectively. After experiments, whole blood clotting time (CT), activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen (FIB) and the activities of a series of coagulation factors and anti-thrombin-III (AT-III) were determined. In the HFD-feeding experiment, metabolic parameters such as blood lipids and glucose were assayed. In parallel experiments, the effect of BBR on inferior vena cava thrombus formation was determined by ligation. Results: The BBR significantly prolonged CT and APTT/PT/TT but decreased FIB both in vitro and in vivo. In addition, BBR significantly increased the activity of AT-III and suppressed the activities of coagulation factors participating in the intrinsic, common and extrinsic coagulation pathways. These BBR-induced changes resulted in a significant inhibition in thrombus formation in the inferior vena cava. In response to HFD-feeding, the rats developed a hypercoagulable state, as indicated by the shortening of CT and APTT/PT/TT, an increase in FIB, overactivation of coagulation factors and the deterioration of thrombosis. After treatment, BBR improved the metabolic parameters of the rats and effectively prevented the HFD-induced blood hypercoagulation. Conclusion: The BBR has anticoagulant activity, which is responsible for its anti-thrombotic effect. The BBR may be suitable for the intervention of hypercoagulability and thrombosis when it is used to treat metabolic diseases.
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How to cite this article:

Can Wang, Yan-Bin Wu, Ai-Ping Wang, Jian-Dong Jiang and Wei-Jia Kong, 2018. Evaluation of Anticoagulant and Antithrombotic Activities of Berberine: A Focus on the Ameliorative Effect on Blood Hypercoagulation. International Journal of Pharmacology, 14: 1087-1098.

DOI: 10.3923/ijp.2018.1087.1098

URL: https://scialert.net/abstract/?doi=ijp.2018.1087.1098

 
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