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International Journal of Pharmacology

Year: 2017 | Volume: 13 | Issue: 4 | Page No.: 403-407
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Authors

Wen- Jie Ng

Ping- Ying Lye

Yek- Jia Chan

Zhi- Khoon Lau

Kah- Yaw Ee

Keywords

Short Communication

Synergistic Effect of Trigona Honey and Ampicillin on Staphylococcus aureus Isolated from Infected Wound

Wen- Jie Ng, Ping- Ying Lye, Yek- Jia Chan, Zhi- Khoon Lau and Kah- Yaw Ee
Objective: The synergistic effect between Trigona honey and ampicillin was studied for antibacterial properties against wound isolated Staphylococcus aureus. Materials and Methods: Clinical isolates of S. aureus were identified biochemically and antibiotic resistance was determined by antibiotic susceptibility test. The clinical isolates and reference strains were treated with Trigona honey, ampicillin and a mixture containing of Trigona honey and ampicillin individually using well diffusion and plate count assays. Scanning electron microscope was used to observe the morphological changes of bacteria tested. Results: A greater anti-staphylococcal activity was observed in the sample with a combination of honey and ampicillin compared to the honey or the ampicillin, with the largest inhibition zone (7.7-18.7 mm) and the highest bactericidal rate (100%). Lysed bacteria and significant morphological alteration on S. aureus were observed in electron micrograph after 24 h incubation with the combination sample. Conclusion: Synergism of Trigona honey and ampicillin exhibited a high degree of antibacterial activity against S. aureus, including antibiotic resistant strains which would be beneficial for the development of wound dressing to prevent and treat wound infection.
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