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Chitooligosaccharides Downregulate TLR4/NF-κB/COX-2 Signaling Cascade in Dextran Sulfate Sodium-treated Mice: A Potential Mechanism for the Anti-colitis Effect |
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Qiaoyun Tong,
Yuanping Yang,
Zhange Xiong,
Zhongyan Li,
Wei Yuan and Ting Wang |
Abstract:
Background and Objective: The anti-inflammatory properties of chitooligosaccharides (COS), a new class of marine-derived natural products and functional foods have been well established, but the underlying mechanisms remain unclear. The aim of the present study is to investigate the effect and potential mechanisms of COS on colonic inflammation in a mouse model of experimental colitis induced by 3.5% Dextran Sulfate Sodium (DSS). Materials and Methods: Animals were randomly divided into 6 groups of 10 mice each: Normal group, model control COS treatment groups: Administered with COS at low (125 mg kg–1), middle (250 mg kg–1) and high (500 mg kg–1) oral administration dose once per day, respectively and positive control group: Salazosulfapyridine 50 mg kg–1. The mRNA and protein expression of toll-like receptor 4 (TLR4), nuclear factor kappa B (NF-κB) and cyclooxygenase (COX)-2 was measured in colon tissues using real-time PCR and immunofluorescence, respectively. Results: Oral administration of COS dose-dependently decreased bloody diarrhea event rate and the Disease Activity Index (DAI), attenuated the loss of body weight, shortening of colon length induced by DSS. Histological damage scores of the colon and colonic prostaglandin (PG)E2 content in DSS-treated mice were reduced by COS, suggesting the preventive effect of COS on the colonic inflammation. Importantly, it was found that the anti-colitis effect shown by COS appear to be related to their capacity to downregulate the expression of TLR4/NF-κB pathway, a canonical proinflamatory signalling, together with its downstream COX-2. Conclusion: The COS administration prevented the DSS-induced activation of TLR4/NF-κB/COX-2 signaling cascade in mouse colon. These finding could give insight into the further evaluation of COS as a food supplement or an alternative agent in the control of ulcerative colitis.
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How to cite this article:
Qiaoyun Tong, Yuanping Yang, Zhange Xiong, Zhongyan Li, Wei Yuan and Ting Wang, 2016. Chitooligosaccharides Downregulate TLR4/NF-κB/COX-2 Signaling Cascade in Dextran Sulfate Sodium-treated Mice: A Potential Mechanism for the Anti-colitis Effect. International Journal of Pharmacology, 12: 720-728.
DOI: 10.3923/ijp.2016.720.728
URL: https://scialert.net/abstract/?doi=ijp.2016.720.728
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