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International Journal of Pharmacology
  Year: 2015 | Volume: 11 | Issue: 4 | Page No.: 394-399
DOI: 10.3923/ijp.2015.394.399
Inhibition of Porcine Reproductive and Respiratory Syndrome Virus in vitro By Forsythoside A
Ming Yang, Yan Lu, Yuanyuan Ma, Guoying Wu, Ross C. Beier, Xiaolin Hou and Guojuan Wu

Abstract:
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) represents a significant challenge to the swine industry worldwide. Current control strategies against PRRSV are still inadequate and there is a need for new antiviral therapy method. Forsythoside is a compound derived from fruit of Forsythia suspensa (Thunb). Vahl suspensa, a traditional antiviral Chinese medicine. The effect of forsythoside on the infection of Marc-145 cells by PRRSV was investigated through the in vitro antivirus action and cytopathic change assay, It was found that forsythoside had a significant protective effect on Marc-145 cells pathological changes, the protective activity from high to low was treatment prior infection, treatment of infected cells, direct virucidal effect, respectively. Furthermore, its effect on the PRRSV RNA capacity and secretion of IFN-α was also evaluated via measurement of the cytopathic changes, real time PCR and ELISA assay. It indicated that forsythpside A inhibited PRRSV RNA synthesis and promoted secretion of IFN-α and in infected Marc-145 cells at a certain concentrations. The research has prepared the ground for later development of forsythoside as an anti-virus agent.
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How to cite this article:

Ming Yang, Yan Lu, Yuanyuan Ma, Guoying Wu, Ross C. Beier, Xiaolin Hou and Guojuan Wu, 2015. Inhibition of Porcine Reproductive and Respiratory Syndrome Virus in vitro By Forsythoside A. International Journal of Pharmacology, 11: 394-399.

DOI: 10.3923/ijp.2015.394.399

URL: https://scialert.net/abstract/?doi=ijp.2015.394.399

 
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