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International Journal of Pharmacology
  Year: 2015 | Volume: 11 | Issue: 4 | Page No.: 372-376
DOI: 10.3923/ijp.2015.372.376
Anti-Inflammatory and Cytotoxic Constituents of Bauhinia retusa
A.M. Al Taweel, A.M. El- Shafae, S. Perveen, G.A. Fawzy and S.I. Khan

Abstract:
Genus Bauhinia (Caesalpiniaceae) is used traditionally for the treatment of wounds, ulcers, diabetes, general pain, inflammation and infections. The aim of the study was to investigate the anti-inflammatory and cytotoxic activities of the bioactive fractions of the leaves of Bauhinia retusa and to identify the chemical constituents in these active fractions by different spectroscopic techniques. The anti-inflammatory activity of chloroform and ethyl acetate soluble fractions (Br-1 and Br-2) of B. retusa and isolated compounds was determined in terms of their ability to inhibit NF-kB and iNOS activity and to decrease oxidative stress in cellular systems. Their cytotoxicity was also determined against a set of 6 mammalian cell lines. Seven compounds were isolated from Br-1 and Br-2, namely methyl gallate, (1) ethyl gallate, (2) kaempferol, (3) quercetin, (4) kaempferol 3-O-α-L-rhamnoside, (5) quercetin 3-O-α-L-rhamnoside (6) and β- sitosterol (7), all reported for the first time from this species. Br-1 and Br-2 were found to decrease oxidative stress and inhibit NF-kB and iNOS activities. Among the isolated compounds, 1-4 were more effective in decreasing oxidative stress and inhibiting iNOS activity than compounds 5-7. None of the compounds inhibited NF-kB. The Br-1 and Br-2 showed cytotoxicity towards KB, SK-OV-3 and LLC-PK1 cell lines. Compounds 1, 2 and 4 were toxic to LLC-PK1 cells. Phytochemical investigation of the leaves of Bauhinia retusa has led to the isolation of seven compounds. Some of which showed promising anti-inflammatory, antioxidant and cytotoxic effects. The reported anti-inflammatory properties of this plant could be attributed to the presence of these constituents.
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How to cite this article:

A.M. Al Taweel, A.M. El- Shafae, S. Perveen, G.A. Fawzy and S.I. Khan, 2015. Anti-Inflammatory and Cytotoxic Constituents of Bauhinia retusa. International Journal of Pharmacology, 11: 372-376.

DOI: 10.3923/ijp.2015.372.376

URL: https://scialert.net/abstract/?doi=ijp.2015.372.376

 
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