Subscribe Now Subscribe Today
Science Alert
FOLLOW US:     Facebook     Twitter
Curve Top
International Journal of Pharmacology
  Year: 2015 | Volume: 11 | Issue: 4 | Page No.: 372-376
DOI: 10.3923/ijp.2015.372.376
Anti-Inflammatory and Cytotoxic Constituents of Bauhinia retusa
A.M. Al Taweel, A.M. El- Shafae, S. Perveen, G.A. Fawzy and S.I. Khan

Genus Bauhinia (Caesalpiniaceae) is used traditionally for the treatment of wounds, ulcers, diabetes, general pain, inflammation and infections. The aim of the study was to investigate the anti-inflammatory and cytotoxic activities of the bioactive fractions of the leaves of Bauhinia retusa and to identify the chemical constituents in these active fractions by different spectroscopic techniques. The anti-inflammatory activity of chloroform and ethyl acetate soluble fractions (Br-1 and Br-2) of B. retusa and isolated compounds was determined in terms of their ability to inhibit NF-kB and iNOS activity and to decrease oxidative stress in cellular systems. Their cytotoxicity was also determined against a set of 6 mammalian cell lines. Seven compounds were isolated from Br-1 and Br-2, namely methyl gallate, (1) ethyl gallate, (2) kaempferol, (3) quercetin, (4) kaempferol 3-O-α-L-rhamnoside, (5) quercetin 3-O-α-L-rhamnoside (6) and β- sitosterol (7), all reported for the first time from this species. Br-1 and Br-2 were found to decrease oxidative stress and inhibit NF-kB and iNOS activities. Among the isolated compounds, 1-4 were more effective in decreasing oxidative stress and inhibiting iNOS activity than compounds 5-7. None of the compounds inhibited NF-kB. The Br-1 and Br-2 showed cytotoxicity towards KB, SK-OV-3 and LLC-PK1 cell lines. Compounds 1, 2 and 4 were toxic to LLC-PK1 cells. Phytochemical investigation of the leaves of Bauhinia retusa has led to the isolation of seven compounds. Some of which showed promising anti-inflammatory, antioxidant and cytotoxic effects. The reported anti-inflammatory properties of this plant could be attributed to the presence of these constituents.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
How to cite this article:

A.M. Al Taweel, A.M. El- Shafae, S. Perveen, G.A. Fawzy and S.I. Khan, 2015. Anti-Inflammatory and Cytotoxic Constituents of Bauhinia retusa. International Journal of Pharmacology, 11: 372-376.

DOI: 10.3923/ijp.2015.372.376






Curve Bottom