Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2015 | Volume: 11 | Issue: 4 | Page No.: 291-299
DOI: 10.3923/ijp.2015.291.299
KB-R7943 Increases Glucose-Stimulated Insulin Secretion from INS-1E Cells through an NCX1-Independent Pathway
Ruth Chu, Ying Ge and Ming Yang

Abstract:
KB-R7943, a Na+/Ca+ exchanger 1 (NCX1) inhibitor, was found to enhance Glucose-Stimulated Insulin Secretion (GSIS) from human and mouse pancreatic islets through inhibiting the forward mode of NCX1. This report studied the effects of KB-R7943 and a different NCX1 inhibitor SN-6, on GSIS from INS-1E cells, a rat pancreatic β-cell line and the potential mechanisms of action. It was found that KB-R7943 significantly enhanced GSIS from INS-1E cells in a concentration-dependent manner. In contrast, no significant effect was observed for SN-6 on GSIS. Similarly, KB-R7943 but not SN-6, enhanced glipizide, a sulfonylurea, stimulated insulin secretion from INS-1E cells. In addition, KB-R7943 but not SN-6, significantly enhanced glipizide-stimulated increase of intracellular Ca2+ ([Ca2+]i). Transient transfection with an NCX1 siRNA resulted in 70 and 62% knockdown of NCX1 gene and protein expression, respectively, in INS-1E cells. Surprisingly, knockdown of NCX1 had little impact on KB-R7943-induced enhancement of glucose- or glipizide-stimulated insulin secretion. Similarly knockdown of NCX1 did not affect KB-R7943 mediated enhancement of glipizide-stimulated increase of [Ca2+]i. Data suggest that the enhancement effect of KB-R7943 on GSIS in INS-1E cells most likely is mediated through an NCX1-independent pathway.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
How to cite this article:

Ruth Chu, Ying Ge and Ming Yang, 2015. KB-R7943 Increases Glucose-Stimulated Insulin Secretion from INS-1E Cells through an NCX1-Independent Pathway. International Journal of Pharmacology, 11: 291-299.

DOI: 10.3923/ijp.2015.291.299

URL: https://scialert.net/abstract/?doi=ijp.2015.291.299

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

Curve Bottom