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International Journal of Pharmacology

Year: 2015 | Volume: 11 | Issue: 2 | Page No.: 114-121
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Authors

Nehal El-Deeb

Mona M. Sharaf

Hala El-Adawi

Keywords

Research Article

Antibacterial and Plasmid Curing Activity of Lactic Acid Bacteria against Multidrug Resistant Bacteria Strains

Nehal El-Deeb, Mona M. Sharaf and Hala El-Adawi
Multiple Drug Resistance (MDR) is a serious health problem and major challenge to global drug discovery programs. Most of the genetic determinants that confer resistance to antibiotics are located on plasmids in bacteria. The present investigation was undertaken to investigate the antibacterial effect and the ability of extra- and intra-cellular extracts of Lactic Acid Bacteria (LAB) to cure plasmid acquiring resistance in certain clinical antibiotic-resistant bacterial isolates (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Shigella sp.). Transformation experiments were carried out using clinical isolates as plasmid donor and Escherichia coli strain HB101 (sensitive to the tested antibiotic), as recipient. Minimal Inhibitory Concentration (MIC) of LAB extracts was determined using the microtiter plate method. Plasmid curing activity of LAB extracts was determined by evaluating the inability of bacterial colonies (pre-treated with LAB extract for 18 h) to grow in the presence of antibiotics. The physical loss of plasmid DNA in the cured derivatives was further confirmed by agarose gel electrophoresis. The LAB showed antibacterial effect, inhibited up to 90% of bacterial biofilm formation and cured the pathogenic bacteria from plasmids. The presence of plasmid in transformants was confirmed through electrophoresis and the transformants were also tested for each antibiotic resistance already recorded for the donor isolates. Both extracts (extra-and intra-cellular extracts) inhibited the growth of the clinical isolates. Extracellular extracts exceeded 90% inhibition on some isolates. The LAB extract mediated plasmid curing resulted in the subsequent loss of antibiotic (Chl, Dox, Ery, Gm, Kaf, Lin and Pen) resistance encoded in the plasmids as revealed by antibiotic resistance profile of cured strains. The extracellular extract of LAB may be a source of anti-plasmid (plasmid borne multiple antibiotic resistance) agents of natural origin.
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