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International Journal of Pharmacology

Year: 2014 | Volume: 10 | Issue: 1 | Page No.: 55-68
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Authors

Juan Yan

Maodong Zheng

Danshen Zhang

Keywords

Research Article

Chrysophanol Liposome Preconditioning Protects against Cerebral Ischemia-reperfusion Injury by Inhibiting Oxidative Stress and Apoptosis in Mice

Juan Yan, Maodong Zheng and Danshen Zhang
Previous studies have indicated that Chrysophanol Liposomes (Chr-lip) have demonstrated potent neuroprotective effects against Cerebral Ischemia Reperfusion (CIR) injury but supporting evidence at molecular mechanisms is lacking. Two hundred healths Kunming male mice were randomly divided into the sham group, model group and Chr-lip treatment (10.0, 5.0 and 0.5 mg kg-1) groups. Chr-lip treatment groups intraperitoneally injected with Chr-lip for three successive days, then subjected to brain ischemia induced by MCAO. After reperfusion 24 h, neurological deficits, brain water content, neuronal ultrastructure, histopathological changes, oxidative stress-related biochemical parameters, neuronal apoptosis and apoptosis-related proteins were assessed by the methods of HE stainning, Hoechst33258 stainning, kits, western blot and real-time quantitative PCR. Chr-lip significantly improved neurological deficits (p<0.05), neuronal ultrastructure and histological deficits, reduced brain water content (p<0.05) and decreased apoptotic cells (p<0.05) after cerebral ischemia. Chr-lip also markedly reduced the level of malondialdehyde (MDA) and enhanced the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in CIR mice (p<0.05). Further investigation demonstrated that Chr-lip remarkably promoted the expression of Bcl-2 and inhibited the expression of Bax, Cytochrome C (Cyt-C) and Caspase3 at protein and mRNA levels (p<0.05), respectively. These findings indicate that Chr-lip has protective effects against CIR injury in mice and the neuroprotective effects may be attributed to attenuating oxidative stress and inhibiting neuronal apoptosis.
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