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International Journal of Pharmacology
  Year: 2014 | Volume: 10 | Issue: 8 | Page No.: 451-460
DOI: 10.3923/ijp.2014.451.460
Berberine Attenuates Isoniazid-Induced Hepatotoxicity by Modulating Peroxisome Proliferator-Activated Receptor γ, Oxidative Stress and Inflammation
Ayman M. Mahmoud, Mousa O. Germoush and Ayman S. Soliman

Abstract: The antituberculosis drug-induced hepatotoxicity is a leading cause of liver injury in many countries. The current study aimed to investigate the possible protective effects of the isoquinoline alkaloid berberine against isoniazid (INH)-induced hepatotoxicity in rats. The experimental rats received 100 mg kg-1 b.wt. INH and concurrently administered berberine at doses of 25 and 50 mg kg-1 orally for 45 days. To evaluate the hepatoprotective effects of berberine, serum markers of the liver function, serum pro-inflammatory cytokines and liver oxidant/antioxidant biomarkers were assayed. In addition, gene expression levels of inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-κB) and peroxisome proliferator-activated receptor gamma (PPARγ) were determined. INH induced hepatic damage evidenced by the significantly elevated serum transaminases, serum pro-inflammatory cytokines and liver lipid peroxidation and nitric oxide levels. Consequently, serum albumin, liver glutathione and activities of the antioxidant enzymes were significantly declined. Further, INH administration produced a significant upregulation of liver iNOS and NF-κB and downregulation PPARγ mRNA expressions. Concurrent supplementation of berberine restored the altered markers to an almost normal state, in a dose-dependent manner. In conclusion , Berberine  protects against INH-induced oxidative stress and inflammation leading to liver injury. The protective effects of berberine can be attributed to its ability to upregulate PPARγ and subsequently suppress NF-κB, iNOS and release of the pro-inflammatory cytokines.
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How to cite this article:

Ayman M. Mahmoud, Mousa O. Germoush and Ayman S. Soliman, 2014. Berberine Attenuates Isoniazid-Induced Hepatotoxicity by Modulating Peroxisome Proliferator-Activated Receptor γ, Oxidative Stress and Inflammation. International Journal of Pharmacology, 10: 451-460.

DOI: 10.3923/ijp.2014.451.460

URL: https://scialert.net/abstract/?doi=ijp.2014.451.460
 
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