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International Journal of Pharmacology

Year: 2014 | Volume: 10 | Issue: 6 | Page No.: 315-321
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Authors

Amany H. Mansour

Rokia Anwer

Ahmad Darwish

Abdulrahman Fahmi Alshaik

Mohamed Mabed

Keywords

Treatment of Acute Promyelocytic Leukemia (APL) with All–Trans Retinoic Acid (ATRA) represents a paradigm in targeted cancer therapy as these drugs cause clinical remission by affecting the stability of the fusion oncoprotein promyelocytic leukemia (PML)/Retinoic Acid Receptor Alpha (RARA). Previous studies have implicated in the main mechanism involved in therapy induced PML/RARA degradation. Investigated the effect of ATRA on the autophagy responses LC3–1, II and Beclin 1 proteins and if ATRA–induced upregulation of autophagy and Beclin 1 in the acute promyelocytic leukemia cells or not. Study included 22 subjects diagnosed as Acute Promyelocytic Leukemia (APL) (M3), also included 20 healthy subjects as a control selected to match the study. Peripheral blood and BM examination, Immunophenotyping, Conventional cytogenetic, Western blotting for LC3-I and LC3-II formation expression and Enzyme Link Immunosorbent Assay (ELISA) kit for Beclin-1 (BECN1). Both beclin-1 and LC3-11 were significantly increased in patient than control group before induction therapy. Proteins induced autophagy LC3-11 was increased after induction therapy than before in APL cells and that indicate autophagic degradation. The present study indicate that the ATRA-induced up regulation of autophagy protein and Beclin-1and LC3-1, 11 in the acute promyelocytic leukemia cells.
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How to cite this article

Amany H. Mansour, Rokia Anwer, Ahmad Darwish, Abdulrahman Fahmi Alshaik and Mohamed Mabed, 2014. All-Trans-Retinoid Acid (ATRA) Induced Upregulation of Autophagy Related Proteins (LC3–1, II and Beclin 1) in Acute Promyelocytic Leukemia. International Journal of Pharmacology, 10: 315-321.

DOI: 10.3923/ijp.2014.315.321

URL: https://scialert.net/abstract/?doi=ijp.2014.315.321

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