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International Journal of Pharmacology

Year: 2013 | Volume: 9 | Issue: 6 | Page No.: 335-347
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Authors

Noura El-Ahmady El-Naggar

Ashraf A. El-Bindary

Noura Salah Nour

Keywords

Research Article

Production of Antimicrobial Agent Inhibitory to some Human Pathogenic Multidrug-Resistant Bacteria and Candida albicans by Streptomyces sp. NEAE-1

Noura El-Ahmady El-Naggar, Ashraf A. El-Bindary and Noura Salah Nour
The production of antimicrobial agent by Streptomyces sp. NEAE-1 in shake flask culture was optimized using response surface methodology. Initial screening of production parameters was performed using a Plackett-Burman design and the variables with statistically significant effects on antimicrobial agent production were identified. Out of the fifteen factors screened, KNO3, NaCl and inoculum size were selected due to significant positive effect on the production of antimicrobial agent. The optimal levels of these variables and the effect of their mutual interactions on antimicrobial agent production were determined using Box-Behnken design. The maximum antimicrobial agent activity was achieved at the KNO3 (3 g L-1), NaCl (0.3 g L-1), inoculum size (4%, v/v). The statistical optimization by response surface methodology resulted in more than one and half-fold increase in the production of antimicrobial agent by Streptomyces sp. NEAE-1. The maximal antimicrobial agent activity is 27 mm inhibition zones which is achieved at the following fermentation conditions: g L-1 (starch 10 g, KNO3 3 g, K2HPO4 0.5 g, NaCl 0.3 g, MgSO4.7H2O 0.5 g, CaCO3 1 g, FeSO4.7H2O 0.01 g), pH 6.5, temperature 25°C, agitation speed (200 rpm min-1), medium volume 50 mL, inoculum size 4% (v/v), inoculum age 60 h and fermentation period 7 days. The value of the coefficient of determination (R2) for the production of antimicrobial agent was 0.955 which indicates a good agreement between experimental and predicted values.
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How to cite this article

Noura El-Ahmady El-Naggar, Ashraf A. El-Bindary and Noura Salah Nour, 2013. Production of Antimicrobial Agent Inhibitory to some Human Pathogenic Multidrug-Resistant Bacteria and Candida albicans by Streptomyces sp. NEAE-1. International Journal of Pharmacology, 9: 335-347.

DOI: 10.3923/ijp.2013.335.347

URL: https://scialert.net/abstract/?doi=ijp.2013.335.347

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