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International Journal of Pharmacology
  Year: 2013 | Volume: 9 | Issue: 5 | Page No.: 288-296
DOI: 10.3923/ijp.2013.288.296
 
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Extraction of Polysaccharides from Saccharomyces cerevisiae and its Immune Enhancement Activity

Hui Wang, Xia Zhang, Pengcheng Dong, Yongjiang Luo and Fusheng Cheng

Abstract:
Microbial polysaccharides are located both inside the fungal cell walls and on the fungal cell surfaces and possess marked immunological properties. In this study, the extraction conditions of Saccharomyces cerevisiae polysaccharides (SCPS) were optimized using response surface methodology and the immune enhancement properties of SCPS were evaluated. The results indicated that the optimal extraction conditions were high pressure homogenization time of 35 min, ultrasonic power of 510 W and ultrasonic time of 26 min, respectively. Under these conditions, the maximal observed value extraction yield of S. cerevisiae polysaccharides (SCPS) was (29.84±0.09)%, which was agreed with predicted value 29.82%. Thin layer chromatography (TLC) exhibited SCPS may be a glucan. Pharmacological experiments indicated that SCPS could increase alkaline phosphatase (AKP), lysozyme (LZM), immunoglobulin A (IgA), immunoglobulin G (IgG) and Epidermal Growth Factor (EGF) levels in serum, increase secreted immunoglobulin A (SIgA) expression in jejunum secretion and decrease prostaglandin E2 (PGE2) expression in colon of immune-compromised rats at medium-dose. Saccharomyces cerevisiae polysaccharides has significant immune enhancement activity and could obviously protect intestine mucosa of immune-compromised rats.
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How to cite this article:

Hui Wang, Xia Zhang, Pengcheng Dong, Yongjiang Luo and Fusheng Cheng, 2013. Extraction of Polysaccharides from Saccharomyces cerevisiae and its Immune Enhancement Activity. International Journal of Pharmacology, 9: 288-296.

DOI: 10.3923/ijp.2013.288.296

URL: https://scialert.net/abstract/?doi=ijp.2013.288.296

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