Submit Manuscript

International Journal of Pharmacology

Year: 2012 | Volume: 8 | Issue: 7 | Page No.: 638-646
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 63

Authors

Min-Woo Hwang

Hyung Woo Kim

Byung Joo Kim

Keywords

Research Article

Involvement of Transient Receptor Potential Melastatin 7 Channels in Orostachys japonicus-induced Apoptosis in Cancer Cells

Min-Woo Hwang, Hyung Woo Kim and Byung Joo Kim
Orostachys japonicus (OJ) herbal preparations have a history of use in Korean folk medicine for the treatment of cancer. The aim of this study was to clarify the potential role of OJ extract on transient receptor potential melastatin 7 (TRPM7) channels in the growth and survival of AGS and MCF-7 cells, the most common human gastric and breast adenocarcinoma cell line. The AGS and MCF-7 cells were treated with varying concentrations of OJ extract. Caspase-3 activity, mitochondrial depolarization and sub-G1 analysis were conducted to determine if AGS and MCF-7 cell death occurs by apoptosis. Also, TRPM7 channels were overexpressed in Human Embryonic Kidney (HEK) 293 cells to identify the role of TRPM7 channels in AGS and MCF-7 cell growth and survival. Addition of OJ extract to the culture medium inhibited AGS and MCF-7 cells growth and survival. Experimental results showed caspase-3 activity, mitochondrial depolarization and sub-G1 were elevated. OJ extract inhibited the TRPM7-like current in AGS and MCF-7 cells and TRPM7 channel overexpressed HEK293 cells by whole cell voltage-clamp recordings. Furthermore, TRPM7 overexpression in HEK 293 cells exacerbated OJ extract-induced cell death. These findings indicate that OJ extract inhibits the growth and survival of gastric and breast cancer cell which is due to the blockade of TRPM7 channel activity. Therefore, OJ extract has an important role in regulation of the survival of gastric and breast cancer cells through TRPM7 channels.
PDF Fulltext XML References Citation

Leave a Comment

Your email address will not be published. Required fields are marked *