Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2012 | Volume: 8 | Issue: 3 | Page No.: 152-160
DOI: 10.3923/ijp.2012.152.160
Protective Effects of the Phenolic Extracts of Fruits against Oxidative Stress in Human Lung Cells
J. Boateng and M. Verghese

Abstract:
Consumption of fruits and the other dietary antioxidants are considered beneficial due to the protection they afford in the pathogenesis associated with oxidative stress. The aim of this study was to evaluate the antioxidative effects of selected fruit extracts (Plums, Apples, Grapes and Cranberries) on human lung fibroblasts (CCD-25LU) exposed to tert-butyl hydroperoxide (tBHP) oxidative stress. Lactate Dehydrogenase (LDH) was used to assess cytotoxicity (cell integrity) and antioxidant enzymes catalase (CAT), glutathione-s-transferase (GST), glutathione peroxidases (GPx) and concentrations of reduced glutathione (GSH) were determined. Results showed that LDH release by cells pretreated with fruits extracts were significantly (p<0.05) lower compared to cells treated with tBHP alone. Antioxidant enzymes (CAT, GST and GPx) in cells pretreated with fruit extracts were increased by 2-4 folds compared to cell exposed to tBHP alone. GSH levels which were significantly (p<0.05) reduced after exposure to tBHP were restored by pretreatment with fruit extracts. Fruits extracts used in this study protected CCD-25LU against oxidative stress induced by tBHP and reduced cell damage. Consumption of fruits may therefore play a significant role in protection against oxidative induced lung diseases.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Preliminary Analysis of in vitro Digestion and Bioactivity Assessment of Basil and Ginger in Human Liver Cancer Cell Line
  •    Synergistic Effects of Glutathione and Vitamin E on ROS Mediated Ethanol Toxicity in Isolated Rat Hepatocytes
  •    Recent Updates on Free Radicals Scavenging Flavonoids: An Overview
  •    Protective Effect of Fresh Apple Skin Extract on Human Umbilical Vein Endothelial Cells Against Glycated Protein-iron Chelate Induced Toxicity
  •    Antioxidative Potential of Ocimum gratissimum and Ocimum canum Leaf Polyphenols and Protective Effects on Some Pro-Oxidants Induced Lipid Peroxidation in Rat Brain: An in vitro Study
How to cite this article:

J. Boateng and M. Verghese, 2012. Protective Effects of the Phenolic Extracts of Fruits against Oxidative Stress in Human Lung Cells. International Journal of Pharmacology, 8: 152-160.

DOI: 10.3923/ijp.2012.152.160

URL: https://scialert.net/abstract/?doi=ijp.2012.152.160

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom