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International Journal of Pharmacology
  Year: 2011 | Volume: 7 | Issue: 2 | Page No.: 263-267
DOI: 10.3923/ijp.2011.263.267
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Antioxidant Properties of Soft Coral Dendronephthya sp.

S. Shahbudin, S. Deny, A.M.T. Zakirun, T.A.H. Haziyamin, B. Akbar John and M. Taher

Present study was conducted to determine the antioxidant property of soft corals belong to the genus: Dendronephthya (Family: Nephtheidae) using α, α-Diphenyl-β-PicrylHydrazyl (DPPH) radical-scavenging and Ferric Thiocyanate (FTC) methods using vitamin E as a positive control. Crude extracts were prepared from 4 Dendronephthya sp. using aqueous, dichoromethane: methanol and methanol extraction. All crude extracts of Dendronephthya sp. exhibited antioxidant properties and the white spots appeared during the rapid screening using Dot-Blot and 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH) staining where the concentration of crude extract was 1.00 g mL-1 against the DPPH concentration of 0.4 mM. In DPPH assay, not all crude extracts showed a significant value in antioxidant activities thus it could be considered as weak free radical species scavenger. The crude extract were diluted to 1000, 500, 250, 125, 63, 31, 15, 7, 4 and 2 μg mL-1, respectively and tested against highly diluted DPPH (0.06 mM). The IC50 for all crude extracts were greater than 1000 μg mL-1. The percentage of free radical scavenging exhibited by the crude extract was at 2 μg mL-1 concentration (the lowest concentration in serial dilution) with 0.81 to 2.89%. Ferric Thiocyanate (FTC) assay, showed absorbance ranges for control, vitamin E and sample were recorded as 0.012-0.858, 0.001-0.315 and 0.001-0.886, respectively. Inhibition percentage of all the crude extract was closer to the control indicated that they are weak lipid peroxidation inhibitor. However, the aqueous extract of species A and C showed higher inhibition percentage from other extract with the percentage value of 10.8 and 10.5% , respectively.
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How to cite this article:

S. Shahbudin, S. Deny, A.M.T. Zakirun, T.A.H. Haziyamin, B. Akbar John and M. Taher, 2011. Antioxidant Properties of Soft Coral Dendronephthya sp.. International Journal of Pharmacology, 7: 263-267.

DOI: 10.3923/ijp.2011.263.267






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