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International Journal of Pharmacology
  Year: 2010 | Volume: 6 | Issue: 2 | Page No.: 97-103
DOI: 10.3923/ijp.2010.97.103
 
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Protective Effect of Squalene on Certain Lysosomal Hydrolases and Free Amino Acids in Isoprenaline-Induced Myocardial Infarction in Rats
K.H.S. Farvin, A. Surendraraj and R. Anandan

Abstract:
This study was aimed to evaluate the preventive role of squalene on free amino acids and lysosomal alterations in experimentally induced myocardial infarction in rats. The levels of lysosomal enzymes (β-glucuronidase, β-galactosidase, β-glucosidase, acid phosphatase and cathepsin D) in plasma and lysosomal fractions, hydroxyproline content and free amino acids in heart tissue were determined. Isoprenaline administration to rats resulted in decreased stability of the membranes which was reflected by significantly (p<0.001) lowered activity of β-glucuronidase, β-galactosidase, β-glucosidase, acid phosphatase and cathepsin D in the lysosomal fraction with a concomitant increase in plasma. Significant (p<0.001) reduction was also observed in the levels of taurine and hydroxyproline with a concomitant increase in the levels of aspartate, glutamate and arginine in the heart tissue of isoprenaline administered rats compared to normal control rats. Pretreatment with squalene at 2% level along with feed for 45 days significantly (p<0.001) prevented these alterations in free amino acids, hydroxyproline and lysosomal hydrolases and maintained the rats at near normal. Thus, the results of this present study show that squalene protects the lysosomal membrane against isoprenaline induced oxidative stress. The overall cardioprotective effect of squalene is ascribable to its ability to strengthen myocardial membrane including lysosomal membrane by its membrane stabilizing action and consequent diminution in the liberation of hydrolytic enzymes or by counteraction of free radicals by its antioxidant nature.
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How to cite this article:

K.H.S. Farvin, A. Surendraraj and R. Anandan, 2010. Protective Effect of Squalene on Certain Lysosomal Hydrolases and Free Amino Acids in Isoprenaline-Induced Myocardial Infarction in Rats. International Journal of Pharmacology, 6: 97-103.

DOI: 10.3923/ijp.2010.97.103

URL: https://scialert.net/abstract/?doi=ijp.2010.97.103

 
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