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International Journal of Pharmacology
  Year: 2010 | Volume: 6 | Issue: 3 | Page No.: 249-256
DOI: 10.3923/ijp.2010.249.256
Molecular Mechanisms of Apoptosis Induced by Scytosiphon gracilis Kogame in HL-60 Cells
Kil-Nam Kim, Young-Min Ham, Min-Seok Yang, Dong Sam Kim, Wook Jae Lee, Nam Ho Lee and Chang-Gu Hyun

Abstract:
Many seaweed species are used as traditional medicine in different parts of the world. However, little data on the anti-cancer effect of seaweed has been published. In this study, we investigated the ability of ethyl acetate extracts of Scytosiphon gracilis Kogame (SGE) to induce apoptosis in cultured human promyelocytic leukemia HL-60 cells. Treatment of HL-60 cells with various concentrations of SGE extracts (12.5-100 μg mL-1) exhibiting IC50 values of 15.68 μg mL-1 resulted in sequences of events marked by apoptosis, such as loss of cell viability, morphology change and internucleosomal DNA fragmentation. The SGE-induced apoptotic cell death was associated with caspase-3 and -9 activation and poly ADP-ribose polymerase degradation in HL-60 cells. This increase in SGE-induced apoptosis was also associated with a reduction in the levels of Bcl-xL, a potent cell-death inhibitor and an increase in levels of the Bax protein, which heterodimerizes with and thereby, inhibits Bcl-2. Present findings suggested that S. gracilis exerts antiproliferative action and growth inhibition on human promyelocytic leukemia HL-60 cells through a mitochondria-dependent apoptotic pathway. Therefore, S. gracilis may have anticancer properties valuable for application in food and drug products.
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How to cite this article:

Kil-Nam Kim, Young-Min Ham, Min-Seok Yang, Dong Sam Kim, Wook Jae Lee, Nam Ho Lee and Chang-Gu Hyun, 2010. Molecular Mechanisms of Apoptosis Induced by Scytosiphon gracilis Kogame in HL-60 Cells. International Journal of Pharmacology, 6: 249-256.

DOI: 10.3923/ijp.2010.249.256

URL: https://scialert.net/abstract/?doi=ijp.2010.249.256

 
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