Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2009 | Volume: 5 | Issue: 5 | Page No.: 298-306
DOI: 10.3923/ijp.2009.298.306
In vitro Cytotoxic Activity of Sargassum thunbergii and Dictyopteris divaricata (Jeju Seaweeds) on the HL-60 Tumour Cell Line
K.N. Kim, Y.M. Ham, J.Y. Moon, M.J. Kim, D.S. Kim, W.J. Lee, N.H. Lee and C.G. Hyun

Abstract:
The cytotoxic activity of a variety of Jeju coastal seaweeds against a panel of tumour cell lines was tested in vitro using a calorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Twenty-three extracts were screened against HL-60 (human promyelocytic leukaemia cell line), HT-29 (human colon carcinoma cell line), B16F10 (murine melanoma cell line) and A549 (human lung cancer cell line). The ethyl acetate (EtOAc) extracts of Sargassum thunbergii and Dictyopteris divaricata showed excellent cytotoxic activity against the HL-60 cell line. Furthermore, the S. thunbergii extract also exhibited good cytotoxic activity against the HT-29 and B16F10 cell lines. To explore the mechanisms of cytotoxicity of S. thunbergii and D. divaricata, we used several measures of apoptosis to determine whether these processes were involved in EtOAc fraction-induced HL-60 cell death. We found that EtOAc fractions induced cell shrinkage, cell membrane blebbing and formation of apoptotic bodies. In addition, HPLC fingerprinting of the S. thunbergii extract revealed that fucosterol was a standard component of these fractions. These results suggest that S. thunbergii and D. divaricata have great potential value as food additives, medicinal supplements for patients with chronic diseases and preventive agents against cancer.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Inhibitory Effect of Sargassum patens on Inflammation and Melanogenesis
  •    Anti-Colon Activity in Ethanolic Extract of Phytolacca americana
  •    Molecular Mechanisms of Apoptosis Induced by Scytosiphon gracilis Kogame in HL-60 Cells
  •    Antimicrobial Activity of Seaweeds Against Multi Drug Resistant Strains
How to cite this article:

K.N. Kim, Y.M. Ham, J.Y. Moon, M.J. Kim, D.S. Kim, W.J. Lee, N.H. Lee and C.G. Hyun, 2009. In vitro Cytotoxic Activity of Sargassum thunbergii and Dictyopteris divaricata (Jeju Seaweeds) on the HL-60 Tumour Cell Line. International Journal of Pharmacology, 5: 298-306.

DOI: 10.3923/ijp.2009.298.306

URL: https://scialert.net/abstract/?doi=ijp.2009.298.306

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom