Subscribe Now Subscribe Today
Science Alert
 
FOLLOW US:     Facebook     Twitter
Blue
   
Curve Top
International Journal of Pharmacology
  Year: 2006 | Volume: 2 | Issue: 3 | Page No.: 280-285
DOI: 10.3923/ijp.2006.280.285
Effects of Sildenafil a Phosphodiesterase 5 Inhibitor on Rat Liver Cell Key Enzymes of Gluconeogenesis and Glycogenolysis
Sheyda Hoseini, Hadi Esmaily , Azadeh Mohammadirad and Mohammad Abdollahi

Abstract:
In the present study, the effects of short-term administration of sildenafil a selective PDE5 inhibitor on serum glucose and hepatic glycogenolysis and gluconeogenesis were examined in rats in vivo. Sildenafil was administered intraperitoneally at doses of 0.5, 1.0 and 5.0 mg kg-1. Two hours post treatment, liver was perfused, removed and homogenized. The activities of the key enzymes of glycogen phosphorylase (GP) and phosphoenolpyruvate carboxykinase (PEPCK) were analyzed in the homogenate. The high dose of sildenafil (5 mg kg-1) caused a significant reduction in serum glucose levels in comparison to control group (94.6±4.7 vs. 121.16±6.05 mg dL-1). The activity of PEPCK remained unchanged when animals were treated with various doses of sildenafil. Interestingly, sildenafil reduced hepatic GP at all doses administered (20.31±1.42, 17.18±1.2, 15.69±1.1 vs. 25.8±1.8 U g-1 liver protein for control group). It is concluded that administration of sildenafil markedly reduces liver glycogenolysis which in turn lowers blood glucose concentration at higher doses. This effect of sildenafil seems to be in relation with its NO mimicking potential and antioxidant properties. The underlying mechanisms involved seem complicated since parallel increase in the activities of GP and PEPCK was not observed.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Stimulation of Hepatic Glycogenolysis and Inhibition of Gluconeogenesis are the Mechanisms of Antidiabetic Effect of Centaurea bruguierana ssp. belangerana
  •    Comparative Improvement in Function of Isolated Rat Langerhans Islets by Various Phosphodiesterase 3, 4 and 5 Inhibitors
  •    Setarud (IMODTM) as a Multiherbal Drug with Promising Benefits in Animal and Human Studies: A Comprehensive Review of Biochemical and Cellular Evidences
  •    Urtica dioica Improves Glucose Control in Diabetes on the Basis of Animal Studies
How to cite this article:

Sheyda Hoseini, Hadi Esmaily , Azadeh Mohammadirad and Mohammad Abdollahi , 2006. Effects of Sildenafil a Phosphodiesterase 5 Inhibitor on Rat Liver Cell Key Enzymes of Gluconeogenesis and Glycogenolysis. International Journal of Pharmacology, 2: 280-285.

DOI: 10.3923/ijp.2006.280.285

URL: https://scialert.net/abstract/?doi=ijp.2006.280.285

 
COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 

       

       

Curve Bottom