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International Journal of Pharmacology
  Year: 2005 | Volume: 1 | Issue: 2 | Page No.: 104-111
DOI: 10.3923/ijp.2005.104.111
 
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Expression of Thymidylate Synthase and Thymidine Phosphorylase as Prognostic Markers in Advanced Esophageal Squamous Cell Carcinoma

Arbabi Bidgoli, Sepideh , Minaee , Bagher , Ghahremani , H. Mohammad , Djamali Zavarhei , Mansoor , Ostad , Seyed Nasser , Fouladdel Shamileh and Azizi, Ebrahim

Abstract:
There are controversial data regarding the correlation between gene expression of Thymidylate Synthase (TS) and Thymidine Phosphorylase (TP) with response to chemotherapy and patients’ survival in gastrointestinal malignancies. Therefore, the prognostic role of TS and TP in GI malignancies and in particular, the Esophageal Squamous Cell Carcinoma (ESCC) needs to be more clarified. The TS and TP status in ESCC was determined and correlated molecular alterations with clinicopathological findings. Tumor samples of 30 surgically resected patients were analyzed by immunohistochemical techniques using primary antibodies for TS and TP and Dako labeled Streptavidin-Biotin® 2 (LSAB2) System detection kit. TS and TP were positive in 63.3 and 89.7% of tumor samples, respectively. Unlike TS, expression of TP was correlated with tumor size (p = 0.013). Although the most prevalent immunophenotype was TS+/TP+ (16/30; 53.33%) but the other immunophenotype (TS-/TP+; 10/30; 33.32%) also showed significant relationship with lymphatic invasion (p = 0.035) and histological differentiation (p = 0.001). Despite low number of metastatic cases, four cases out of five had positive expression of both TS and TP in their primary tumor samples. The high TP expression (89.1%) and its established role in tumor angiogenesis could be the reason for its correlation with tumor size and also progression of early stage of ESCC to the advanced stage. Based on present data as well as others, the overexpression of both TS and TP would indicate a poor prognosis for patients with ESCC. On the other hand, our data showed that 36.7 and 10.3% of patients were TS and TP negative, respectively, that obviously affect their response to 5-FU based chemotherapy. In conclusion, the present data showed the importance of TS and TP as valuable prognostic markers that are in correlation with clinicopathological findings.
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How to cite this article:

Arbabi Bidgoli, Sepideh , Minaee , Bagher , Ghahremani , H. Mohammad , Djamali Zavarhei , Mansoor , Ostad , Seyed Nasser , Fouladdel Shamileh and Azizi, Ebrahim , 2005. Expression of Thymidylate Synthase and Thymidine Phosphorylase as Prognostic Markers in Advanced Esophageal Squamous Cell Carcinoma. International Journal of Pharmacology, 1: 104-111.

DOI: 10.3923/ijp.2005.104.111

URL: https://scialert.net/abstract/?doi=ijp.2005.104.111

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