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International Journal of Oceanography and Marine Ecological System
  Year: 2014 | Volume: 3 | Issue: 1 | Page No.: 1-8
DOI: 10.3923/ijomes.2014.1.8
 
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Isolation and Characterization of Exopolysaccharide Producing Bacteria from Pak Bay (Mandapam)

Parthiban Karuppiah, Vignesh Venkatasamy and Thirumurugan Ramasamy

Abstract:
Bacterial exopolysaccharides possess a wide variety of properties that may not be found in more traditional polymers of plant or algal or animal origin. In the present study, the exopolysaccharide producing bacteria was isolated and characterized from coastal (Mandapam) area of Pak bay, Tamilnadu, India. Soil samples were collected and the exopolysaccharide producing strains were screened and characterized by 16s rDNA sequencing method. The effect of different carbon sources on exopolysaccharide production was examined. Then the exopolysaccharide was synthesized and characterized by calorimetric, IR and HPLC method. Calorimetric analysis of exopolysaccharide reveals the composition of exopolysaccharide and in the IR analysis the band at 1385 to 1380 cm-1 indicates the presence of C-H stretching. An absorbance at 1730 cm-1 indicating the presence of carboxyl group. In addition, a small absorption at 1550 cm-1 indicating the presence of either amino sugars or proteins were present in exopolysaccharide. Exiguobacterium sp. was isolated and it produces considerable amount of exopolysaccharide when the medium was supplied with sucrose.
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How to cite this article:

Parthiban Karuppiah, Vignesh Venkatasamy and Thirumurugan Ramasamy, 2014. Isolation and Characterization of Exopolysaccharide Producing Bacteria from Pak Bay (Mandapam). International Journal of Oceanography and Marine Ecological System, 3: 1-8.

DOI: 10.3923/ijomes.2014.1.8

URL: https://scialert.net/abstract/?doi=ijomes.2014.1.8

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