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International Journal of Dairy Science

Year: 2018 | Volume: 13 | Issue: 1 | Page No.: 30-39
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Authors

Lamiaa Ibrahim Ahmed

Lamiaa Ibrahim Ahmed

Karima Mogahed Fahim

Keywords

Research Article

Incidence of Subclinical Mastitis with Special Reference to Lactate Dehydrogenase (LDH) Enzyme as a Biomarker

Lamiaa Ibrahim Ahmed Lamiaa Ibrahim Ahmed's LiveDNA and Karima Mogahed Fahim
Background and Objective: Subclinical mastitis is considered as one of the most costly diseases in dairy production throughout the world and the second problem in dairy Egyptian farms after laminitis, therefore this study focused on determination of the prevalence of subclinical mastitis (SCM) in two dairy herds located in Ismailia and El-Sharkia governorate, Egypt with special reference to Lactate Dehydrogenase (LDH) enzyme as a biomarker with isolation of some of the etiological agents. Materials and Methods: About 230 quarter milk samples (QMS) were collected from apparently healthy 60 cows and subjected to California Mastitis Test (CMT). The positive CMT samples were subjected to some of the screening tests (Milk Somatic Cell Count [MSCC], Koestler value and Lactate Dehydrogenase (LDH) enzyme level in milk and blood serum samples and isolation and identification of some of the etiological agents. Result: The CMT revealed that 43.5% of the examined samples had subclinical mastitis, the mean values of Milk Somatic Cell Count [MSCC], Koestler value and Lactate Dehydrogenase (LDH) enzyme level in milk and blood serum samples were 1.1×106±61554.12 cells mL–1, 4.62±0.08, 621.15±55.45 and 633.17±30.20 U L–1, respectively. Bacteriological examination showed the isolation of Streptococci and Coliforms from 88 and 100% of the examined samples, respectively, Polymerase Chain Reaction (PCR) revealed the isolation of Sreptococcus agalactiae and E. coli strains with percentages of 21.74 and 1.36%, respectively. Conclusion: The poor management and udder health practices and inadequate screening tests applying by most farmers in the study area resulted in high prevalence rate of SCM with isolation of pathogenic E. coli.
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