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International Journal of Dairy Science

Year: 2017 | Volume: 12 | Issue: 1 | Page No.: 41-46
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Authors

Jhy- Ming Li

Shu- Yu Hsieh

Yan- Ming Horng

Chean- Ping Wu

Keywords

Research Article

Genotyping of the CSN1S1 Locus in Taiwan Dairy Goats using PCR-RFLP and AS-PCR

Jhy- Ming Li, Shu- Yu Hsieh, Yan- Ming Horng and Chean- Ping Wu
Objective: This study aimed to investigate αs1-casein (CSN1S1) gene polymorphisms in Taiwan dairy goats by using PCR-restriction fragment length polymorphism (PCR-RFLP) in combination with allele-specific-PCR (AS-PCR). Methodology: One hundred and sixty Taiwan dairy goats were used to detect the presence of CSN1S1 gene polymorphisms. After DNA amplification, the PCR products were digested with XmnI restriction enzyme or were directly subjected to gel electrophoresis. Results: The following 11 CSN1S1 genotypes were identified: AA, AE, AF, A01, EE, EF, EN, E01, FF, F01 and N01 with frequencies of 0.13, 0.13, 0.29, 0.02, 0.02, 0.19, 0.01, 0.03, 0.13, 0.04 and 0.01, respectively. Among the CSN1S1 alleles, the frequency of the F allele (0.40) was the highest, indicating that the CSN1S1 F allele was the most common allele in all examined Taiwan dairy goats. Conclusion: In conclusion, a quick and accurate technique was used for typing goats independently of CSN1S1 genotype. By examining CSN1S1 gene polymorphisms, we could identify breeds carry a high percentage of homozygous CSN1S1 F allele for use in the fresh milk industry in Taiwan.
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