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International Journal of Dairy Science
  Year: 2017 | Volume: 12 | Issue: 4 | Page No.: 282-288
DOI: 10.3923/ijds.2017.282.288
Polymorphisms in Genes Encoding Kappa Casein Milk Protein in Dairy Cattle
Trini Susmiati , Prabowo Purwono Putro, Sarmin , Claude Mona Airin, Hartiningsih and Pamungkas Bagus Satria

Abstract:
Background and Objective: Polymorphisms of kappa-casein protein genes are closely related to the quality of milk production and composition. The purpose of the study was to investigate polymorphisms in genes encoding kappa-casein (κ-casein) milk protein produced by the dairy cattle of KP4/PIAT UGM Yogyakarta and BBPTU-HPT Baturraden. Materials and Methods: Twenty-three cattle were used to investigate kappa-casein polymorphisms in dairy cows from two different animal farms. Nine cattle were distributed from BBPTU-HPT Baturraden, Central Java and 14 cattle were allocated from KP4/PIAT UGM Yogyakarta. The research procedures included blood sampling, DNA extractions and DNA amplification using Polymerase Chain Reaction (PCR). Kappa-casein gene PCR products were analyzed by using the PCR-RFLP technique with restriction enzymes Hinf I and Hind III. Results: The results of restriction digestion using the Hinf I showed four restriction fragments in the blood sample from BPPTU-HPT Baturraden and three restriction fragments from KP4/PIAT UGM Yogyakarta. The digestion of PCR product using Hind III produced one restriction fragment in the blood samples from KP4/PIAT UGM Yogyakarta and 4 fragments in the samples from BPPTU-HPT Baturraden. Conclusion: The dairy cattle of BPPTU Baturraden offer a more favorable genetic selection of animals for milk production and industry due to their genetic variants in terms of genotypes and alleles.
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How to cite this article:

Trini Susmiati, Prabowo Purwono Putro, Sarmin , Claude Mona Airin, Hartiningsih and Pamungkas Bagus Satria, 2017. Polymorphisms in Genes Encoding Kappa Casein Milk Protein in Dairy Cattle. International Journal of Dairy Science, 12: 282-288.

DOI: 10.3923/ijds.2017.282.288

URL: https://scialert.net/abstract/?doi=ijds.2017.282.288

 
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