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International Journal of Dairy Science

Year: 2015 | Volume: 10 | Issue: 5 | Page No.: 228-235
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Authors

J. Otte

M. Abdel-Hamid

A. Osman

Keywords

Research Article

Comparative Assessment of Peptide Concentration in Milk Protein Hydrolysates and Fractions

J. Otte, M. Abdel-Hamid and A. Osman
A comparative study using the Kjeldahl method and two colorimetric methods for the determination of total protein/peptide concentrations in a range of intact and hydrolysed milk protein was carried out. The samples were goat whey, camel whey, buffalos milk retentate and hydrolysates of these made with different enzymes as well as hydrolysate fractions. Bicinchoninic acid (BCA) and o-phthaldialdehyde (OPA) were used as coloring agents to specifically react with proteins and peptides. The results obtained with the BCA method and bovine serum albumin as standard did not correlate with the results obtained by the Kjeldahl method (r2 = 0.17). The OPA method showed better agreement with the Kjeldahl method (r2 = 0.69) and with the use of a whey protein hydrolysate as standard good estimates of the peptide contents in the hydrolysed milk protein samples was obtained compared to the results obtained with the Kjeldahl method.
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