Production of β-galactosidase using Novel Yeast Isolate from Whey

Research Article

Production of β-galactosidase using Novel Yeast Isolate from Whey

Shweta Kumari, Parmjit S. Panesar and Reeba Panesar

The hydrolysis of lactose by enzyme β-galactosidase into glucose and galactose play an important role in biotechnology, pharmaceutical and food processing industries. The aim of present study was focused on the isolation of novel yeast strains and formulation of low-cost medium based on whey for optimal β-galactosidase activity. β-galactosidase activities of isolated strains were carried out by ONPG assay. The yeast isolate (Kluyveromyces marxianus) showed maximum enzyme activity (1710 IU g^-1 DW), when grown on whey permeate at pH 5.5, temperature 30°C after incubation period of 28 h. The utilization of dairy waste (whey) was found to be economical for β-galactosidase production by using isolated yeast cells. This enzyme is also known for its trans-galactosylation reaction and synthesized lactose based derivatives including lactulose and galacto-oligosaccarides, which can be further used in probiotic foods.

PDF Fulltext XML References Citation

How to cite this article

Shweta Kumari, Parmjit S. Panesar and Reeba Panesar, 2011. Production of β-galactosidase using Novel Yeast Isolate from Whey. International Journal of Dairy Science, 6: 150-157.

DOI: 10.3923/ijds.2011.150.157

URL: https://scialert.net/abstract/?doi=ijds.2011.150.157

International Journal of Dairy Science

Article Trend

Total views 100

Authors

Shweta Kumari

Parmjit S. Panesar

Reeba Panesar

Keywords

Research Article

Production of β-galactosidase using Novel Yeast Isolate from Whey

How to cite this article

Related Articles

Leave a Reply