• [email protected]
  • +971 507 888 742
Submit Manuscript
SciAlert
  • Home
  • Journals
  • Information
    • For Authors
    • For Referees
    • For Librarian
    • For Societies
  • Contact
  1. International Journal of Dairy Science
  2. Vol 2 (1), 2007
  3. 90-94
  • Online First
  • Current Issue
  • Previous Issues
  • More Information
    Aims and Scope Editorial Board Guide to Authors Article Processing Charges
    Submit a Manuscript

International Journal of Dairy Science

Year: 2007 | Volume: 2 | Issue: 1 | Page No.: 90-94
DOI: 10.3923/ijds.2007.90.94

Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend



Total views 159

Authors


Othman E. Othman


Sahar Ahmed


Keywords


  • Goat
  • kappa-casein
  • milk protein
  • PCR-RFLP
Research Article

Genotyping of the Caprine Kappa-casein Variants in Egyptian Breeds

Othman E. Othman and Sahar Ahmed
This study aimed to detect the genetic variants of kappa-casein (k-CN) in four breeds of goat reared in Egypt using PCR-RFLP technique. Three primers 13F, kb1 and kb2 with two restriction enzymes Alw44I and HaeIII have been used for genotyping k-CN exon 4. The PCR product -resulted from kb1and kb2 primers- with size 459 bp was digested by Alw44I endonuclease to detect A or B and C alleles. The 645 bp fragment resulted from PCR with kb2 and 13F primers was digested by HaeIII for detecting A and E alleles. Allele B was identified by combining the results of digestion with Alw44I and HaeIII endonucleases. The results showed that the allele B is the most common allele in four tested breeds and appeared at a frequency 68.2%, whereas allele C was appeared with 22.7%. Allele A was displayed in different frequencies ranged from 10 to 45.5%. The appearance of each allele was in homozygous or heterozygous genotypes. Allele E was not present in all tested animals. Identification of the genotypes of Egyptian goat milk protein will enable us to establish better strategy for breeding the Egyptian goat contributed in filling the nutritious gap in milk production.
PDF Fulltext XML References Citation

How to cite this article

Othman E. Othman and Sahar Ahmed, 2007. Genotyping of the Caprine Kappa-casein Variants in Egyptian Breeds. International Journal of Dairy Science, 2: 90-94.

DOI: 10.3923/ijds.2007.90.94

URL: https://scialert.net/abstract/?doi=ijds.2007.90.94

Leave a Comment


Your email address will not be published. Required fields are marked *

Useful Links

  • Journals
  • For Authors
  • For Referees
  • For Librarian
  • For Socities

Contact Us

Office Number 1128,
Tamani Arts Building,
Business Bay,
Deira, Dubai, UAE

Phone: +971 507 888 742
Email: [email protected]

About Science Alert

Science Alert is a technology platform and service provider for scholarly publishers, helping them to publish and distribute their content online. We provide a range of services, including hosting, design, and digital marketing, as well as analytics and other tools to help publishers understand their audience and optimize their content. Science Alert works with a wide variety of publishers, including academic societies, universities, and commercial publishers.

Follow Us
© Copyright Science Alert. All Rights Reserved