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International Journal of Cancer Research
  Year: 2018 | Volume: 14 | Issue: 2 | Page No.: 58-69
DOI: 10.3923/ijcr.2018.58.69
 
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CD74 a Potential Therapeutic Target for Breast Cancer Therapy: Interferon Gamma Up-regulates its Expression in CAMA-1 and MDA-MB-231 Cancer Cells

Waleed Alabdulmonem , Homaidan T. Alhomaidan, Zafar Rasheed, Inamul Hasan Madar, Noura Alasmael, Shaza Alkhatib and Hussain Al Ssadh

Abstract:
Background and Objective: Cluster of differentiation (CD) 74 is well known for its diverse proinflammatory and immunological functions. This study was undertaken to investigate the role of interferon (IFN)-γ on CD74 expression in breast cancer cells. Materials and Methods: The CAMA-1 and MDA-MB-231 cancer cells were stimulated with IFN-γ and expression of CD74 was determined by flow cytometry and western immunoblotting using Raji cells as a positive control. The MTT assays were used to determine the effect of IFN-γ on the proliferation and migration of cells. Whereas, cell surface expression of HLA-ABC and HLA-DR were acquired by flow cytometry. Results: Treatment of CAMA-1 and MDA-MB-231cells with IFN-γ significantly increased the expression of CD74. Not only have these, IFN-γ also stimulates the expression of HLA-ABC and HLA-DR in a dose-dependent manner in CAMA-1 and MDA-MB-231cells. Moreover, IFN-γ treatment also inhibited the proliferation and migration of CAMA-1 and MDA-MB-231cells. Conclusion: The IFN-γ up-regulates CD74 expression in human breast cancer cells. The results concluded that IFN-γ played a key role in modulating the expression of CD74, as well as in the proliferation and the migration of CAMA-1 and MDA-MB-231 cancer cells. Data suggest that CD74 plays a key role in tumor immunogenicity as well as in breast cancer immunoediting in humans.
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How to cite this article:

Waleed Alabdulmonem, Homaidan T. Alhomaidan, Zafar Rasheed, Inamul Hasan Madar, Noura Alasmael, Shaza Alkhatib and Hussain Al Ssadh, 2018. CD74 a Potential Therapeutic Target for Breast Cancer Therapy: Interferon Gamma Up-regulates its Expression in CAMA-1 and MDA-MB-231 Cancer Cells. International Journal of Cancer Research, 14: 58-69.

DOI: 10.3923/ijcr.2018.58.69

URL: https://scialert.net/abstract/?doi=ijcr.2018.58.69

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