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International Journal of Cancer Research
  Year: 2016 | Volume: 12 | Issue: 3-4 | Page No.: 152-161
DOI: 10.3923/ijcr.2016.152.161
Expression of Vascular Endothelial Growth Factor Protein in Both Serum Samples and Excised Tumor Tissues of Breast Carcinoma Patients
Halla Mohamed Ragab, HebatAllah Mohamed Shaaban, Nabila Abd El Maksoud, Samah Mohamed Radwan, Wafaa Abd Elaziz and Nesreen Hassan Hafez

Abstract:
Background and Objective: This study examined the correlation between immunohistochemistry (IHC) expression of the Vascular Endothelial Growth Factor (VEGF) in primary breast carcinoma tissue and its concentration in blood samples of the same patients preoperatively and to the other established clinicopathological parameters namely, tumor size, grade, lymph node status, Estrogen Receptor (ER), Progesterone Receptor (PR) status and human epidermal growth factor receptor 2 (Her2-neu) score. The current study also stressed on whether the ELISA detection is more sensitive and effective in diagnosis of breast cancer. Materials and Methods: Serum concentration of VEGF was measured using Enzyme Linked Immune Sorbent Assay (ELISA) in 92 primary breast cancer patients and compared with 25 benign breast disease patients. Also, tissue expression of VEGF was measured by immunohistochemistry. Results: Serum VEGF levels were significantly elevated in patients with malignant breast cancer (p = 0.000). The median serum level in the malignant group was 579 pg mL–1 and in the control group 200 pg mL–1. On the other hand no correlation was found between concentrations of serum VEGF and clinicopathological parameters. A significant association was showed between VEGF expression and lymph node metastasis, tumor size more than 2 cm and Her2-neu status (p<0.0001). No significant association was found between VEGF and patient age, histology, grade, ER and PR status (p>0.05). All positive cases for VEGF with strong positivity (score ++) were grade 2 and 3. Conclusion: The VEGF is overexpressed in breast carcinomas compared to the benign breast disease. Tissue expression of VEGF can be used as a prognostic marker due to the significant association with the large tumor size, lymph node metastasis and positive Her2-neu status. Tissue expression of VEGF can be used as a prognostic marker due to the significant association with the large tumor size, lymph node metastasis and positive Her2-neu status.
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How to cite this article:

Halla Mohamed Ragab, HebatAllah Mohamed Shaaban, Nabila Abd El Maksoud, Samah Mohamed Radwan, Wafaa Abd Elaziz and Nesreen Hassan Hafez, 2016. Expression of Vascular Endothelial Growth Factor Protein in Both Serum Samples and Excised Tumor Tissues of Breast Carcinoma Patients. International Journal of Cancer Research, 12: 152-161.

DOI: 10.3923/ijcr.2016.152.161

URL: https://scialert.net/abstract/?doi=ijcr.2016.152.161

 
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