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International Journal of Cancer Research
  Year: 2016 | Volume: 12 | Issue: 3-4 | Page No.: 140-151
DOI: 10.3923/ijcr.2016.140.151
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Modifying Effects of Pistachio Nuts on Antioxidant Enzymes in Azoxymethane (AOM)-induced Formation of Aberrant Crypt Foci
J. Boateng, R. Miller-Cebert, L. Shackelford and M. Verghese

Background and Objectives: There is limited information on the effect of pistachio nuts in cancer prevention. Since pistachios contain several protective compounds with antioxidants properties, the aim of this study is to determine if pistachios can reduce precancerous colon cancer lesions in rats by affecting several biomarkers of oxidative stress including induction of endogenous antioxidant enzymes. Methodology: Thirty Fisher 344 male rats were randomly assigned to 5 groups. Rats in groups 1 and 2 were fed AIN-93G as positive (CON+) and negative control (CON-), while rats in groups 3-5 were assigned AIN-93G with 5, 10 and 15% pistachio meal (PM). Rats (except group 2), received AOM injections at 7 and 8 weeks of age and killed at 17 weeks of age by CO2 asphyxiation. Results: Total ACF and crypt multiplicity, antioxidant enzymes: Glutathione-s-transferase (GST), glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) were determined. Total serum cholesterol (TC) and triglycerides (TG) were also assessed. Results showed significant (p<0.05) reductions in ACF in all treatment groups compared to the rats fed CON+(158). The ACF ranged from 72-119 in rats fed 5, 10 and 15% PM. Rats in group 2 developed no ACF. Significant (p<0.05) increase in antioxidant enzyme activities (protein μmol mg–1) was observed compared to CON+. The GST (5.86-10.84), GR (4.91-7.21), CAT (1.66-3.38), GPx (4.25-7.61) and SOD (protein U mg–1) (1.02-1.73). The TC and TG were significantly (p<0.05) decreased in the treatment groups compared to CON+. Data indicated PM reduced ACF by enhancing phase II and antioxidant enzyme activities and reducing serum lipids. Conclusion: The PM could be investigated as putative dietary chemoprevention in colon cancer therapy.
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  •    A Comparison of Rice Bran, Corn Oil and Soybean Oil Against Azoxymethane Induced Colon Cancer in a Fisher 344 Rat Model
  •    Resveratrol, a Phytoalexin Enhances Hepatic Antioxidant Defense in 1,2-dimethylhydrazine-induced Colon Carcinogenesis
How to cite this article:

J. Boateng, R. Miller-Cebert, L. Shackelford and M. Verghese, 2016. Modifying Effects of Pistachio Nuts on Antioxidant Enzymes in Azoxymethane (AOM)-induced Formation of Aberrant Crypt Foci. International Journal of Cancer Research, 12: 140-151.

DOI: 10.3923/ijcr.2016.140.151








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