Synergistic Effects of Green Tea Catechin and Phytic Acid Increases the Cytotoxic Effects on Human Colonic Adenocarcinoma Cell Lines


	International Journal of Cancer Research
	Year: 2012 \| Volume: 8 \| Issue: 2 \| Page No.: 49-62 DOI: 10.3923/ijcr.2012.49.62

Synergistic Effects of Green Tea Catechin and Phytic Acid Increases the Cytotoxic Effects on Human Colonic Adenocarcinoma Cell Lines

J. Khatiwada, S. Davis and L.L. Williams

Abstract: Colorectal cancer is sensitive to dietary intervention. Epidemiological data suggest that high intake of fruits and vegetables may decrease colorectal cancer risk. This experiment was designed to study the preventive effect of green tea catechins and phytic acid in cell culture against the colon tumors. Selected concentrations (0.25 to 25 mM) of green tea catechins and phytic acid were used to treat Caco-2 and HT29 cells maintained in Dulbecco’s modified Eagles Medium (DMEM) with 10% fetal bovine serum. For assay 5x10⁵ cells/well/100 μL were seeded to a 24 well culture plate and incubated at 37°C and 7% CO₂ until monolayer was developed, then 400 μL of fresh serum-free DMEM was added to the 24 wells. At the same time selected concentrations (0.25-25 mM) of green tea catechins and phytic acid made up to 100 μL with saline was added and incubated for 24 and 48 h to determine the cytotoxic effect in term of Lactate Dehydrogenase (LDH), Alkaline Phosphatase (AP) and apoptosis assay. The results of this experiment showed that, when Caco-2 and HT29 cells were treated with various concentrations (0.25-25 mM) of green tea catechins and phytic acid for 24 and 48 h, the LDH and AP release was dose and time dependent. When HT29 cells treated with 25 mM concentration of green tea catechins for 24 h and combination of green tea catechins and phytic acid at 5 mM concentration for 12 h showed signs of membrane blebbing which indicates that singly and/or in combination of green tea catechins and phytic acid showed the anti-tumorigenesis activity. Thus, green tea catechins and phytic acid makes it promising chemo-preventive agents of colon cancer.

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How to cite this article:

J. Khatiwada, S. Davis and L.L. Williams, 2012. Synergistic Effects of Green Tea Catechin and Phytic Acid Increases the Cytotoxic Effects on Human Colonic Adenocarcinoma Cell Lines. International Journal of Cancer Research, 8: 49-62.

DOI: 10.3923/ijcr.2012.49.62

URL: https://scialert.net/abstract/?doi=ijcr.2012.49.62

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