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International Journal of Cancer Research

Year: 2009 | Volume: 5 | Issue: 3 | Page No.: 123-129
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Authors

A.F.A. Aisha

S.A. Alrokayan

K.M. Abu-Salah

Y. Darwis

A.M.S. Abdul Majid

Keywords

Research Article

In vitro Cytotoxic and Apoptotic Properties of the Stem Bark Extract of Sandoricum koetjape on Breast Cancer Cells

A.F.A. Aisha, S.A. Alrokayan, K.M. Abu-Salah, Y. Darwis and A.M.S. Abdul Majid
Both cytotoxic and apoptotic properties of the stem bark extract of Sandoricum koetjape were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on three breast cancer cell lines and one normal cell line; MCF-7, MDA-MB-231, T47D and MCF-10A, respectively. Caspase Glo 3/7 assay was used to study the apoptotic activity on MCF-7 as a model cell line. The n-hexane extract showed a dose dependent growth inhibition of all tested cell lines with IC50 values between 44 and 48 μg mL-1. At 100 μg mL-1, the extract induced apoptotic cell death of MCF-7 by inducing activity of the effector caspases 3 and 7. These results indicated that n-hexane extract of S. koetjape has cytotoxic and apoptotic properties on breast cancer cell lines making it a good candidate for further studies.
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