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International Journal of Cancer Research

Year: 2009 | Volume: 5 | Issue: 3 | Page No.: 105-114
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Authors

A.F.A. Aisha

H.B. Sahib

K.M. Abu-Salah

Y. Darwis

A.M.S. Abdul Majid

Keywords

Cytotoxic and anti-angiogenic properties of n-hexane extract of Sandoricum koetjape stem bark were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro- 5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on Human Umbilical Vein Endothelial Cell (HUVEC) and both colon cancer and normal cell lines; HCT-116, HT-29 and CCD-18CO. Rat aorta ring assay was used to study the anti-angiogenic properties of the extract. At 100 μg mL-1, the extract showed 94±5.5% inhibition of the outgrowth of the blood vessels from the rat aorta rings. The extract also showed a dose dependent growth inhibition of all tested cell lines, IC50 values against HCT-116, HUVEC, CCD-18CO and HT-29 were 14, 23, 50 and 52 μg mL-1, respectively. At 50 μg mL-1, the extract had potently induced apoptotic cell death of HCT-116 colon cancer cell line by inducing caspases 3 and 7 activity. These results showed that n-hexane extract of S. koetjape possess both anti-angiogenic and apoptotic properties on colon cancer cell lines making it a good candidate for further studies.
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Comments

Martin Chandra Putra Manurung Reply

Good Morning...
My nationality is a citizen of Indonesia...
Could you mind giving or suggesting to me another journal about bark sandoricum koetjape..?
I'm still making a thesis for my scholar...
Thank you

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