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International Journal of Cancer Research
  Year: 2008 | Volume: 4 | Issue: 3 | Page No.: 81-91
DOI: 10.3923/ijcr.2008.81.91
 
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Tamoxifen and Melatonin Differentially Influence Apoptosis of Normal Mammary Gland Cells: Ultrastructural Evidence and p53 Expression

N.E. Abdelmeguid, M.H. Mostafa, A.M. Abdel-Moneim, A.F. Badawi and N.S. Abou Zeinab

Abstract:
The mammary gland cells of Sprague Dawley rats treated with melatonin and/or tamoxifen were analyzed for apoptosis and ultrastructural damage using transmission electron microscopy. The alterations of p53 expression were determined by western blotting. The experiment lasted for 8 weeks where animals were subcutaneously injected with melatonin (0.5 mg/rat daily) and/or tamoxifen (2.5 mg/rat twice a week). It is of considerable interest that the doses used were equivalent to human therapeutic doses reported previously. Obvious features of apoptosis due to melatonin treatment were noticed. These included irregularities of nuclear shapes, clumping and margination of nuclear chromatin and dilatation of the nuclear envelope forming nuclear blebs. Electron micrographs of tamoxifen treated mammary gland showed that there were occasional irregularities in nuclear shape and chromatin distribution. Melatonin/tamoxifen administration resulted in normal cellular and nuclear morphology with normal chromatin distribution. In addition, pyknotic cells increased in frequency, yet this value was less compared to melatonin treated group as well as tamoxifen treated group. On the other hand, melatonin resulted in insignificant increase of p53 expression compared to control while tamoxifen treatment revealed p53 expression similar to control group. In melatonin/tamoxifen treated group, p53 expression was almost confounded greatly to control group. It could be concluded that the synergetic interaction between melatonin and tamoxifen on the expression of p53 is weakly evident on normal mammary gland tissue.
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How to cite this article:

N.E. Abdelmeguid, M.H. Mostafa, A.M. Abdel-Moneim, A.F. Badawi and N.S. Abou Zeinab, 2008. Tamoxifen and Melatonin Differentially Influence Apoptosis of Normal Mammary Gland Cells: Ultrastructural Evidence and p53 Expression. International Journal of Cancer Research, 4: 81-91.

DOI: 10.3923/ijcr.2008.81.91

URL: https://scialert.net/abstract/?doi=ijcr.2008.81.91

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