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International Journal of Cancer Research
  Year: 2006 | Volume: 2 | Issue: 4 | Page No.: 420-423
DOI: 10.3923/ijcr.2006.420.423
Evaluating Homogeny of Cellular Mitotic Cytosolic Protein Antigens of Chinese Hamster Ovary (CHO), HeLa, Vero and Human Lymphocytes
Maddaly Ravi , Deepa Parvathi , V. Govind Pai , M. Preetha , B. Sulogna Ghosh and Solomon F.D. Paul

Abstract:
Mitotic factors peak at the G2-mitotic transition and have no species specificity. Altered cell cycle dynamics are hallmarks of cancers and this leads us to believe in altered protein profiles in cancerous conditions distinct from normal cells. Antiserum raised against CHO cytosolic mitotic protein antigens was tested with cytosolic interphase and mitotic protein extracts of HeLa, Vero and normal human lymphocytes. The mitotic and interphase extracts of transformed cells showed distinct reactions with the antibodies. Constitutively expressed proteins, particularly, those in the higher molecular weight range presumably contribute to the cross reactivity. Neither mitotic nor interphase extracts of normal human lymphocytes reacted with the antiserum, indicating the homogeneity of cytosolic mitotic protein antigens among cancerous cell lines and their shared epitope matching. The possible applications of this are in areas including cancer immunotherapy; for the detection and selective destruction of such cells in a population without disturbing normal cell functions.
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How to cite this article:

Maddaly Ravi , Deepa Parvathi , V. Govind Pai , M. Preetha , B. Sulogna Ghosh and Solomon F.D. Paul , 2006. Evaluating Homogeny of Cellular Mitotic Cytosolic Protein Antigens of Chinese Hamster Ovary (CHO), HeLa, Vero and Human Lymphocytes. International Journal of Cancer Research, 2: 420-423.

DOI: 10.3923/ijcr.2006.420.423

URL: https://scialert.net/abstract/?doi=ijcr.2006.420.423

 
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