Subscribe Now Subscribe Today
Science Alert
 
Blue
   
Curve Top
International Journal of Cancer Research
  Year: 2006 | Volume: 2 | Issue: 1 | Page No.: 31-41
DOI: 10.3923/ijcr.2006.31.41
 
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Level of the Expression of VEGF-A, B, C, D and their Receptors (FLT-1, KDR and FLT-4) and its Correlation with Prognosis in Patients with Colorectal Cancer

Khaled A. Rmali , Malcolm C.A. Puntis and Wen G. Jiang

Abstract:
This study examined, qualitatively and quantitatively, the expression of VEGFs and their receptors, in a group of patients with colorectal cancer and thier correlation to tumour progression. Human colorectal cancer tissues (n = 48) and normal background tissues (n = 48) were obtained after surgery. RNA was extracted from frozen sections for gene amplification. The expression of VEGF-A, B, C and D and their respective receptors VEGFR-1, VEGFR-2 and VEGFR-3 (Flt-1, KDR and FLT-4) were assessed using RT-PCR and the quantity of their transcripts were determined using real-time-quantitative PCR (Q-RT-PCR). VEGF-B (p = 0.001), VEGF-C (p = 0.02), VEGFR-1(p = 0.019) and VEGFR-2 (p = 0.005) were significantly raised in colon cancer tissues compared with the levels detected in normal background tissues. The expression of VEGF-A, VEGF-D and VEGF-R3 in cancer tissues was not statistically significant (p>0.05) from background tissues. The level of the expression of VEGF-C and VEGF-R3 showed no difference in Dukes B and Dukes C. Patients who had cancer penetrating into and through the muscularis propria of the bowel wall and developed nodal involvement (Dukes C), exhibited significantly (p<0.05) higher levels of VEGF-B and VEGFR-2 compared with patients who were node negative (Dukes A and B). We conclude that here is aberrant expression of angiogenic factors VEGF-B and VEGF-C, together with their respective receptors VEGF-R1 and VEGF-R2 (FLT-1 and KDR) in colon cancer compared to normal colon tissues. The high level of expression of VEGF-B and VEGF-R2 were correlated to tumour invasion and nodal involvement (Dukes C) and therefore may have prognostic and therapeutic values in colon cancer patients.
PDF Fulltext XML References Citation Report Citation
 RELATED ARTICLES:
  •    Prevalence of p53 and p21 Expression in Colorectal Cancer: A Histopathologic Study from Iran
  •    AT1 Receptors Activation Enhances the Expression of MMP-2, MMP-13 and VEGF but not MMP-9 in B16F10 Melanoma Cells
How to cite this article:

Khaled A. Rmali , Malcolm C.A. Puntis and Wen G. Jiang , 2006. Level of the Expression of VEGF-A, B, C, D and their Receptors (FLT-1, KDR and FLT-4) and its Correlation with Prognosis in Patients with Colorectal Cancer. International Journal of Cancer Research, 2: 31-41.

DOI: 10.3923/ijcr.2006.31.41

URL: https://scialert.net/abstract/?doi=ijcr.2006.31.41

COMMENT ON THIS PAPER
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

Curve Bottom