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International Journal of Biological Chemistry

Year: 2017 | Volume: 11 | Issue: 1 | Page No.: 17-22
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Authors

Dixon Dhawan

Jeena Gupta

Jeena Gupta

Keywords

Research Article

Comparison of Different Solvents for Phytochemical Extraction Potential from Datura metel Plant Leaves

Dixon Dhawan and Jeena Gupta Jeena Gupta's LiveDNA
Background: The bioactive components present in the Datura metel plant are known to be responsible for its medicinal properties, to cure many diseases like asthma and bronchitis. However, the extraction method for these bioactive components is not yet standardized. The present study was undertaken to compare the effect of using different extraction solvents to extract the active components like alkaloids, flavinoids, saponins, steroids and tannins from the dried leaves of the Datura plant. Methodology: To achieve this, different extracts from the plant leaves were made using soxhlet apparatus. The extraction solvents used were distilled water, methanol, acetone, chloroform, ethyl acetate and hexane. Phytochemical estimations, total phenol concentration, flavonoid concentration and antioxidant activity have been evaluated to compare the efficiency of different extraction solvents. Results: The results shows that using methanol as an extraction solvent works best for the extraction of various active phytochemicals with flavonoid concentration of 16.48±0.22 mg of quercetin equivalent/100 g of extract, phenol concentration of 8.493±0.21 mg gallic acid equivalent/100 g of extract and 41.2±0.64% 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition which is significantly higher than chloroform which results in the minimum extraction with flavonoid concentration of 6.71±0.44 mg of quercetin equivalent/100 g of extract, phenol concentration of 0.196±0.02 mg gallic acid equivalent/100 g of extract and 15.3% 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition. Conclusion: To best of our knowledge this is the first report that directly compares the 6 extraction solvents for the extraction of active components from the Datura plant leaves and shows that methanol should be the solvent of choice.
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Dixon Dhawan and Jeena Gupta, 2017. Comparison of Different Solvents for Phytochemical Extraction Potential from Datura metel Plant Leaves. International Journal of Biological Chemistry, 11: 17-22.

URL: https://scialert.net/abstract/?doi=ijbc.2017.17.22

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