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International Journal of Biological Chemistry
  Year: 2017 | Volume: 11 | Issue: 1 | Page No.: 1-8
DOI: 10.3923/ijbc.2017.1.8
Combination of Spices Aqueous Extracts as Antioxidant and Novel Anticancer Agents in Human Liver Cancer Cell Line
Manal M. Ramadan, Amr F. Mansour, Reda M. Fekry, Marwa T. Salem, Fathy M. Mahaya, Mamdouh M. Ali and Noha S. Mohamed

Abstract:
Objective: The present study investigated the synergistic effect and the antioxidant activity of 7 aqueous extracts from Egyptian spices; identified the phenolics and flavonoids constituents of two different blends prepared from these extracts using DART-MS for the first time and also evaluated the cytotoxic effect of the blends on liver cancer cell line Hep G2. Methodology: The antioxidant activity of the water extracts was evaluated using 2,2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2- azinobis (3- ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and Ferric Reducing Antioxidant Power (FRAP) assays. Based on folin-ciocalteu and AlCl3-colorimetric methods, total phenolic content and total flavonoids of the extracts were assayed. The separation and identification of phenolics/flavonoids constituents of extracts blends were performed using direct analysis in real time DART-mass spectrometry. In vitro cytotoxicity was performed against liver human cancer cell line (Hep G2) using MTT assay and 5-flurouracilas a reference drug. Results: Clove/cinnamon extracts combination showed synergistic effect in promoting the cytotxicity of Hep G2 cancer cells as well as scavenging DPPH radicals, while combination had antagonistic effect. Phenolic constituents e.g., catechol, pyrogallol, eugenol, linalool, caffeic and ferulic acids were identified in both combination using DART-MS technique, however, flavonoids e.g., pinocembrin, catechin and apeginin were detected only in clove/cinnamon combination which may correlated to its synergistic effect. Conclusion: Aqueous extract of clove/cinnamon blend demonstrated a synergistic effect as an anticancer or antioxidant agent, while clove/cinnamon/thyme blend, although showed antagonistic effect as an antioxidant but exhibited good cytotoxic effect on liver cancer cell line Hep G2. These findings may provide some basis for the purported synergistic effects of traditional Egyptian spices and facilitate their utilization in combination as functional foods and dietary supplements.
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How to cite this article:

Manal M. Ramadan, Amr F. Mansour, Reda M. Fekry, Marwa T. Salem, Fathy M. Mahaya, Mamdouh M. Ali and Noha S. Mohamed, 2017. Combination of Spices Aqueous Extracts as Antioxidant and Novel Anticancer Agents in Human Liver Cancer Cell Line. International Journal of Biological Chemistry, 11: 1-8.

DOI: 10.3923/ijbc.2017.1.8

URL: https://scialert.net/abstract/?doi=ijbc.2017.1.8

 
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