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International Journal of Biological Chemistry
  Year: 2010 | Volume: 4 | Issue: 4 | Page No.: 190-202
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Screening of Chemical Analysis, Antioxidant Antimicrobial and Antitumor Activities of Essential Oil of Oleander (Nerium oleander) Flower

H.F.M. Ali, F.M.A. El-Ella and N.F. Nasr

In this study, the GC/MS analysis and in vitro screening of antioxidant antimicrobial and antitumor activities of essential oil extracted from Oleander flower (Nerium oleander) were investigated. The GS/MS analysis recorded 64 components account 94.69% of the total essential oil were composition. Amount of total phenolics was 136.54±3.32 mg as gallic acid/g essential oil. The antioxidant activity was studied by three methods (DPPH assay; β-Carotene/linoleic acid a bleaching assay and ferric reducing power assay). Oleander essential oil had significantly antioxidant activity compared to synthetic antioxidants (trolox and BHT). Antitumor activity was tested as ability of inhibition the growth of ehrlich ascites carcinoma cells line and obtained result indicated gradually increase of antitumor activity with increasing of oil concentration. Antimicrobial activity was observed by agar disc diffusion technique against different strains of Gram-positive; Gram-negative; yeast and mold. The essential oil displayed a variable degree of antimicrobial activity against the different strains tested compared with that of the standard antibiotics tested and the Minimum Inhibition Concentration (MIC) values ranged from 125 to 500 and 250 to 2000 μg mL-1 for bacteria and fungi respectively. The toxicity of oleander essential oil was studied in animal model system by different parameters including LD50 ; diarrhea; (GPT) activity; lactate dehydrogenase (LDH) activity and createnine levels. Toxicity results indicated that no adverse effect recorded with all concentrations of oleander oil range used in present study.
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How to cite this article:

H.F.M. Ali, F.M.A. El-Ella and N.F. Nasr, 2010. Screening of Chemical Analysis, Antioxidant Antimicrobial and Antitumor Activities of Essential Oil of Oleander (Nerium oleander) Flower. International Journal of Biological Chemistry, 4: 190-202.


29 June, 2010
Suleiman Umar:
It is a good work,worthy of continuing further to know the constituent of the oil that has antitumor action and its mode of action,but i need to know the unit used in MIC.
14 December, 2010

Dear friends: ¡Congratulations to yours and the other researchers whom are looking for new properties and treatments from the mother nature who is wounded by ourself. have ever knew that plant was poison. The medical literature spoke about many people died, but sometimes in the life "the poison save of the poison" Could be safe any water stem destillation from flowers of Nerium Oleander. What are the security measures to distill this plant and its risks?. Thank you Fer

30 June, 2013
So happy finding this research as I use and promote neriumad. I read that oleander with cancer cells creates oxidative stress and inflammation. But now see here research that says it has anti oxidants, etc.....what a glorious relief! .♡ Keep up the great research.
29 August, 2014
Monica Chmiel:
i too have heard from others the danger of this plant. i too am a distributor of NeriumA.D. This article made me feel better about selling this. Thank you!
Monica Chmiel



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