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International Journal of Biological Chemistry
  Year: 2008 | Volume: 2 | Issue: 1 | Page No.: 1-13
 
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Reactive Oxygen Species Damaged Hemoglobin Presents Unique Epitopes for Type 1 Diabetes Autoantibodies

Zafar Rasheed, Rizwan Ahmad, Naila Rasheed, Trivendra Tripathi and Rashid Ali

Abstract:
The study hypothesized that oxidative by-product like hydrogen peroxide (H2O2), would reacts with hemoglobin (Hb) and help to initiate autoimmunity in patients with type 1 diabetes. To test this hypothesis, Hb was modified by reactive oxygen species (ROS). Modification incurred in Hb was characterized by physico-chemical techniques. ROS-modified Hb was found to be highly immunogenic in rabbits as compare to native Hb. The binding characteristics of circulating autoantibodies in type 1 diabetes patients against native and ROS-modified Hb were assessed. Diabetes patients (n = 39) were examined by direct binding ELISA and their results were compared with healthy age-matched controls (n = 22). High degree of specific binding by 48% of patient`s sera towards ROS-modified Hb, in comparison to its native analogue (p<0.05) was observed. Competitive inhibition ELISA reiterates the direct binding results. Collectively, ROS caused extensive damage to Hb altering its immunogenicity, the neo-epitopes, thus generated might play a role in the induction of circulating autoantibodies in type 1 diabetes mellitus.
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How to cite this article:

Zafar Rasheed, Rizwan Ahmad, Naila Rasheed, Trivendra Tripathi and Rashid Ali, 2008. Reactive Oxygen Species Damaged Hemoglobin Presents Unique Epitopes for Type 1 Diabetes Autoantibodies . International Journal of Biological Chemistry, 2: 1-13.

URL: https://scialert.net/abstract/?doi=ijbc.2008.1.13

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