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International Journal of Biological Chemistry
  Year: 2007 | Volume: 1 | Issue: 1 | Page No.: 69-74
DOI: 10.3923/ijbc.2007.69.74
 
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Comparative Studies on the Antimicrobial Activity of Leaf Extract from Ocimum basilicum and Antagonistic Activity of Isolates from Refuse on ome Selected Pathogens

Oladunmoye M. Kolawole

Abstract:
Refuse samples were screened for the presence of resident bacteria and fungi antagonistic to the growth of some pathogens. Nine bacteria and four fungi were isolated. The pathogens used are clinical isolates which include; Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus. The assay was carried out using the agar well diffusion method and the plugging method for the bacteria and fungi isolates respectively. Seven bacteria were antagonistic to at least a pathogen and all the fungi isolates showed antagonistic activity against the five pathogens at varying degrees. A 60% ethanol extract from the leaf of Ocimum basilicum (Labiatae) was investigated for in vitro antimicrobial activities against the same set of pathogens. The results showed no antimicrobial activity at concentration of 50, 100 and 200 mg mL-1 while at 300 mg mL-1 there were activities against all the pathogens except Staphylococcus aureus. Bacillus cereus and Salmonella typhi were found to be the most susceptible to the extract. Compared to the plant extracts, none of the bacteria isolated from the refuse showed antagonistic activity against Klebsiella pneumoniae. Actinomyces isolate was the most active of the bacteria isolates and the activity is comparable to that of the leaf extract of Ocimum basilicum. Penicillium isolates has the strongest activity of the fungi isolates and it has a broader zone of inhibition and spectrum of activity than the leaf extract.
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How to cite this article:

Oladunmoye M. Kolawole , 2007. Comparative Studies on the Antimicrobial Activity of Leaf Extract from Ocimum basilicum and Antagonistic Activity of Isolates from Refuse on ome Selected Pathogens. International Journal of Biological Chemistry, 1: 69-74.

DOI: 10.3923/ijbc.2007.69.74

URL: https://scialert.net/abstract/?doi=ijbc.2007.69.74

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