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International Journal of Biological Chemistry
  Year: 2007 | Volume: 1 | Issue: 3 | Page No.: 179-183
 
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Antibiotics Assay Pattern of Escherichia coli Isolates from Rainwater in Ondo State, Nigeria

F.C. Akharaiyi , T.T. Adebolu , D.E. Akponmendaye and F.O. Omoya

Abstract:
Twenty rainwater samples each were randomly collected from four geographical zones of Ondo State, Nigeria, which fall under a rain zone of 1200 mm annually. Eosin methylene blue agar was used to isolate Escherichia coli from the water, while nutrient agar was used for enumeration of total bacterial count and violet red bile lactose agar for Coliform bacilli. Escherichia coli isolates from the rainwater samples were subjected to antibiotic assay. Among the rainwater samples collected from each zone, Ondo State South had the highest (28.72%) E. coli occurrence. Also, the E. coli isolates from this zone were found less sensitive and resistant in most cases to the antibiotics. The average mean E. coli counts recovered from the rainwater samples ranged between 4.50-4. 80 (log cfu m-1). This count is above the recommended value (0/100 mL) in water quality by WHO standard. The E. coli isolates showed various degree of sensitivity to the antibiotics potency ranging from 0->10 mm diameter inhibitory halo. Meanwhile, nitrofurantoin, augmentin and amoxylin had very low potency on the isolated E. coli with 0 and 25%, respectively, while others had high potency of between 75-100% on the isolates.
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How to cite this article:

F.C. Akharaiyi , T.T. Adebolu , D.E. Akponmendaye and F.O. Omoya , 2007. Antibiotics Assay Pattern of Escherichia coli Isolates from Rainwater in Ondo State, Nigeria. International Journal of Biological Chemistry, 1: 179-183.

URL: https://scialert.net/abstract/?doi=ijbc.2007.179.183

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