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International Journal of Botany
  Year: 2018 | Volume: 14 | Issue: 1 | Page No.: 30-35
DOI: 10.3923/ijb.2018.30.35
Genotypic Variation Affects the Efficiency of the Genetic Transformation Process in Balanites aegyptiaca
Ibrahim Abdullah Alaraidh

Abstract:
Background and Objective: Balanites aegyptiaca, belonging to family Zygophyllaceae, is multipurpose tree considered highly drought-tolerant but salt-sensitive and cultivated in several arid and tropical regions in North and west Africa and West Asia. The plant parts contain several secondary metabolites and there is a high oil content in the kernels already used in biodiesel production. This study was aimed at investigating effect of the genotypic variation in B. aegyptiaca plants (collected from different geographical locations, Riyadh, Saudi Arabia and Halayeb, Egypt) on the efficiency of the genetic transformation process for both ERD10 and nptII genes. Materials and Methods: Two B. aegyptiaca genotypes collected from two different geographical regions with different ecological conditions (Halayeb, Egypt and Riyadh, Saudi Arabia) were used in this study. The genetic transformation was performed using Agrobacterium tumefacienss train GV3101 harboring the binary vector pBinAR containing ERD10 (Early Responsive to Dehydration 10) and nptII genes, to produce salt-tolerant B. aegyptiaca plants. Results: The results showed that each genotype collected from the different geographical locations after transformation exhibited significant differences in the number of leaves per regenerated explant and shoot length, where by nodal explants from Halayeb, Egypt exhibited the highest values. Conclusion: Current findings emphasize the impact of the genotypic variation (geographical location) of the used plant on the efficiency of the genetic transformation process.
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How to cite this article:

Ibrahim Abdullah Alaraidh , 2018. Genotypic Variation Affects the Efficiency of the Genetic Transformation Process in Balanites aegyptiaca. International Journal of Botany, 14: 30-35.

DOI: 10.3923/ijb.2018.30.35

URL: https://scialert.net/abstract/?doi=ijb.2018.30.35

 
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