Submit Manuscript

International Journal of Botany

Year: 2011 | Volume: 7 | Issue: 2 | Page No.: 195-199
Facebook Twitter Digg Reddit Linkedin StumbleUpon E-mail

Article Trend

Total views 171

Authors

Homa Mahmoodzadeh

Elham Esparham

Keywords

Research Article

Changes in Hydrogen Peroxide Content and Antioxidant Enzymes in Abscisic Acid-induced Antioxidant Defense in Leaves of Bean Seedlings

Homa Mahmoodzadeh and Elham Esparham
The plant hormone abscisic acid (ABA), as a stress signal, plays important roles in the regulation of plant responses to environmental stresses. The relationship among abscisic acid (ABA) accumulation, the generation of Reactive Oxygen Species (ROS) and the activities of several antioxidant enzymes such as Catalase (CAT), Ascorbate Peroxidase (APX) and Glutathione Reductase (GR) was investigated in leaves of detached bean (Phaseolus vulgaris L.) plants exposed to 10,100 and 1000 μM ABA [Detached leaves of bean were cut into leaf segments and floated in solutions containing 0, 10, 100 and 1,000 μM ABA, at 25°C and then used for assay of antioxidant enzymes]. Treatment with 10 and 100 μM ABA significantly increased the levels of O2¯ and H2O2, followed by an increase in activities of CAT, APX and GR in leaves of bean seedlings. Treatment with 1,000 μM ABA led to a more abundant generation of O2¯and H2O2 .The activities of these antioxidative enzymes were reduced when compared with the treatment of 100 μM ABA. These results indicate that treatment with low concentrations of ABA (10 to 100 μM) induced an antioxidative defense response against oxidative damage, but a high concentration of ABA (1,000 μM) induced an excessive generation of ROS and led to an oxidative damage in plant cells.
PDF Fulltext XML References Citation

Related Articles

Effects of Spermine and Abscisic acid on growth and Biochemical contents of Vigna mungo L. under high temperature and salt-stress
Effect of VAM on Drought Tolerance and Growth of Plant in Comparison with the Effect of Growth Regulators
Exogenous Glucose and Abscisic Acid Pre-treatment in Indica Rice (Oryza sativa L. spp. indica) Responses to Sodium Chloride Salt Stress
The Effect of Plant Growth Regulators and Temperature Shock on 1AA-oxidase Activity of Roots and Endogenous ABA Level of Leaves
Effects of Cold Acclimation and Exogenous Pytohormone Abscisic Acid Treatment on Physiological Indicators of Winterness Wheat

Leave a Comment

Your email address will not be published. Required fields are marked *